ArticleThe plant pathology journal2016
Natural Occurrence of Tomato leaf curl New Delhi virus in Iranian Cucurbit Crops.
Article in The plant pathology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 45 citations in OpenAlex.
- Co-infection and recombination-driven emergence of begomovirus complex causing mosaic disease in muskmelon (Cucumis melo L.).Molecular biology reports · 2026Article
- The Insidious Threat: Assessing the Dangers and Spread of Tomato Leaf Curl New Delhi Virus.The plant pathology journal · 2025Review
- Targeting vacuolar ATPase in tobacco whitefly,Journal of biosciences · 2025Review
- Cy-1, a major QTL for tomato leaf curl New Delhi virus resistance, harbors a gene encoding a DFDGD-Class RNA-dependent RNA polymerase in cucumber (Cucumis sativus).BMC plant biology · 2024Article
- A comprehensive review onFrontiers in genetics · 2024Review
- Article
- The haplotype-resolved T2T reference genome highlights structural variation underlying agronomic traits of melon.Horticulture research · 2023Article
- Tomato leaf curl New Delhi virus: an emerging plant begomovirus threatening cucurbit production.aBIOTECH · 2023Review
- An Insight into Emerging Begomoviruses and their Satellite Complex causing Papaya Leaf Curl Disease.Current genomics · 2023Review
- Article
- Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy.Plants (Basel, Switzerland) · 2020Article
- Natural Hosts and Genetic Diversity of the Emerging Tomato Leaf Curl New Delhi Virus in Spain.Frontiers in microbiology · 2019Article
- Tomato Leaf Curl New Delhi Virus: An Emerging Virus Complex Threatening Vegetable and Fiber Crops.Viruses · 2017Review
- The Prediction of a New CLCuD Epidemic in the Old World.Frontiers in microbiology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The main areas for field-grown vegetable production in Iran were surveyed during the years of 2012-2014 to determine the occurrence of begomoviruses infecting these crops. A total of 787 leaf samples were collected from vegetables and some other host plants showing virus-like symptoms and tested by an enzyme-linked immunosorbent assay (ELISA) using polyclonal antibodies produced against Tomato yellow leaf curl virus (TYLCV). According to the ELISA results, 81 samples (10.3%) positively reacted with the virus antibodies. Begomovirus infections were confirmed by polymerase chain reaction (PCR) using previously described TYLCV-specific primer pair TYLCV-Sar/TYLCV-Isr or universal primer pair Begomo-F/Begomo-R. The PCR tests using the primer pair TYLCV-Sar/TYLCV-Isr resulted in the amplification of the expected fragments of ca. 0.67-kb in size for ELISA-positive samples tested from alfalfa, pepper, spinach and tomato plants, confirming the presence of TYLCV. For one melon sample, having a week reaction in ELISA and no reaction in PCR using TYLCV-specific primers, the PCR reaction using the primer pair Begomo-F/Begomo-R resulted in the amplification fragments of the expected size of ca. 2.8 kb. The nucleotide sequences of the DNA amplicons derived from the isolate, Kz-Me198, were determined and compared with other sequences available in GenBank. BLASTN analysis confirmed the begomovirus infection of the sample and showed 99% identities with Tomato leaf curl New Delhi virus (ToLCNDV); phylogenetic analysis supported the results of the database searches. This study reports the natural occurrence of TYLCV in different hosts in Iran. Our results also reveal the emergence of ToLCNDV in Iranian cucurbit crops.
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