Evidence map›Paper›PMID 27298243›Full record

ArticleComparative medicine2016

Effects of Murine Norovirus on Chlamydia pneumoniae-Accelerated Atherosclerosis in ApoE(-/-) Mice.

Karuna Patil, Lee Ann Campbell, Michael E Rosenfeld, Jisun Paik, Thea Brabb, Kevin D O'brien, Lillian Maggio-Price, Charlie C Hsu

Abstract read
PubMed Full text at PMC
In one paragraph

Article in Comparative medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 5 citations in OpenAlex.

  1. Pooled it
  2. Guidance on Establishing Health Monitoring Panels for Laboratory Mice to Reflect Contemporary Pathogen Prevalence.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025
    Review
  3. Infections withFrontiers in aging neuroscience · 2025
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Karuna PatilDepartments of Comparative Medicine, University of Washington, Seattle, Washington, USA kpatil@uw.edu.
Lee Ann CampbellDepartments of Epidemiology, University of Washington, Seattle, Washington, USA.
Michael E RosenfeldDepartments of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, USA.
Jisun PaikDepartments of Comparative Medicine, University of Washington, Seattle, Washington, USA.
Thea BrabbDepartments of Comparative Medicine, University of Washington, Seattle, Washington, USA.
Kevin D O'brienDepartments of Medicine, University of Washington, Seattle, Washington, USA.
Lillian Maggio-PriceDepartments of Comparative Medicine, University of Washington, Seattle, Washington, USA.
Charlie C HsuDepartments of Comparative Medicine, University of Washington, Seattle, Washington, USA.
University of Washington · US

Funding

PILOT STUDY--CLINICAL NUTRITION RESEARCHP30DK035816 · NIDDK · UNIVERSITY OF WASHINGTON · PI Ellen A Schur · 1986 to 2026
$30.4M
Effects of Murine norovirus infection on Mouse Models of AtherosclerosisR21OD011135 · OD · UNIVERSITY OF WASHINGTON · PI MAGGIO-PRICE, LILLIAN · 2012 to 2012
$232k
NIDDK NIH HHS P30 DK035816NIH HHS R21 OD011135
6 · The paper itself

Abstract

Chlamydia pneumoniae (Cpn), a common respiratory pathogen of humans, is associated with human cardiovascular disease and the acceleration of atherosclerosis in hyperlipidemic animal models. Our laboratory has demonstrated that murine norovirus (MNV), a prevalent infection of laboratory mice, can unpredictably alter atherosclerosis in hyperlipidemic Ldlr(-/-) and ApoE(-/-) mice. Given that MNV has a tropism for macrophages and may exacerbate atherogenesis, we investigated whether coinfection with MNV and Cpn might alter macrophage phenotypes in vitro and atherosclerosis in ApoE(-/-) mice. In the presence of oxidized low-density lipoprotein, coinfection of ApoE(-/-) bone marrow-derived macrophages (BMDM) with MNV and Cpn resulted in significant increases in gene expression of IL6, MCP1, iNOS, and TNFα compared with Cpn-monoinfected BMDM. On the basis of these findings, we hypothesized that concurrent MNV-Cpn infection might increase plaque lesion size in vivo. As expected, Cpn monoinfection of ApoE(-/-) mice increased mean plaque size by 62% compared with that in uninfected mice. However, MNV did not significantly alter plaque lesion size in MNV-Cpn-coinfected mice compared with Cpn-monoinfected mice. There were no differences in aortic cytokines locally at the site of plaque development or in peritoneal macrophages at 1 wk after infection in MNV-Cpn-coinfected mice compared with Cpn-monoinfected mice. MNV was not detected in the aortic tissue of MNV-infected mice at 1 or 8 wk after infection regardless of Cpn status. These data suggest that MNV infection does not appreciably alter plaque development in Cpn-accelerated atherosclerosis in ApoE(-/-) mice.

Indexed as

AnimalsApolipoproteins EAtherosclerosisCaliciviridae InfectionsChemokine CCL2Chlamydophila pneumoniaeCoinfectionInterleukin-6MiceNitric Oxide Synthase Type IINorovirusPneumonia, BacterialTumor Necrosis Factor-alphaApolipoproteins EChemokine CCL2Interleukin-6interleukin-6, mouseNitric Oxide Synthase Type IINos2 protein, mouseTumor Necrosis Factor-alpha

Identifiers

PMID27298243
PMCPMC4907527
OpenAlexW2470754277

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.