Evidence map›Paper›PMID 27297678›Full record

ArticlePlant physiology2016

Saturating Light Induces Sustained Accumulation of Oil in Plastidal Lipid Droplets in Chlamydomonas reinhardtii.

Hugh Douglas Goold, Stéphan Cuiné, Bertrand Légeret, Yuanxue Liang, Sabine Brugière, Pascaline Auroy, Hélène Javot, Marianne Tardif, Brian Jones, Fred Beisson and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 72 citations in OpenAlex.

  1. Knock Down ofPlants (Basel, Switzerland) · 2025
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  14. Autophagy Induced Accumulation of Lipids inFrontiers in plant science · 2021
    Article
  15. The MicroalgaPlant physiology · 2020
    Article
  16. Article
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  19. Endoplasmic reticulum acyltransferase with prokaryotic substrate preference contributes to triacylglycerol assembly inProceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Hugh Douglas GooldCommissariat à l'Energie Atomique, Centre National de la Recherche Scientifique Aix Marseille Université, Unité Mixte de Recherche 7265, Institut de Biosciences et Biotechnologies, Cadarache 13108, France (H.D.G., S.C., B.L., Y.L., P.A., H.J., F.B., G.P., Y.L.-B.);Faculty of Agriculture and the Environment, University of Sydney, Sydney, New South Wales 2006, Australia (H.D.G., B.J.); andCommissariat à l'Energie Atomique, INSERM, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble 38000, France (S.B., M.T.).
Stéphan CuinéCommissariat à l'Energie Atomique, Centre National de la Recherche Scientifique Aix Marseille Université, Unité Mixte de Recherche 7265, Institut de Biosciences et Biotechnologies, Cadarache 13108, France (H.D.G., S.C., B.L., Y.L., P.A., H.J., F.B., G.P., Y.L.-B.);Faculty of Agriculture and the Environment, University of Sydney, Sydney, New South Wales 2006, Australia (H.D.G., B.J.); andCommissariat à l'Energie Atomique, INSERM, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble 38000, France (S.B., M.T.).ORCID 0000-0002-3000-3355
Bertrand LégeretCommissariat à l'Energie Atomique, Centre National de la Recherche Scientifique Aix Marseille Université, Unité Mixte de Recherche 7265, Institut de Biosciences et Biotechnologies, Cadarache 13108, France (H.D.G., S.C., B.L., Y.L., P.A., H.J., F.B., G.P., Y.L.-B.);Faculty of Agriculture and the Environment, University of Sydney, Sydney, New South Wales 2006, Australia (H.D.G., B.J.); andCommissariat à l'Energie Atomique, INSERM, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble 38000, France (S.B., M.T.).
Yuanxue LiangCommissariat à l'Energie Atomique, Centre National de la Recherche Scientifique Aix Marseille Université, Unité Mixte de Recherche 7265, Institut de Biosciences et Biotechnologies, Cadarache 13108, France (H.D.G., S.C., B.L., Y.L., P.A., H.J., F.B., G.P., Y.L.-B.);Faculty of Agriculture and the Environment, University of Sydney, Sydney, New South Wales 2006, Australia (H.D.G., B.J.); andCommissariat à l'Energie Atomique, INSERM, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble 38000, France (S.B., M.T.).
Sabine BrugièreCommissariat à l'Energie Atomique, Centre National de la Recherche Scientifique Aix Marseille Université, Unité Mixte de Recherche 7265, Institut de Biosciences et Biotechnologies, Cadarache 13108, France (H.D.G., S.C., B.L., Y.L., P.A., H.J., F.B., G.P., Y.L.-B.);Faculty of Agriculture and the Environment, University of Sydney, Sydney, New South Wales 2006, Australia (H.D.G., B.J.); andCommissariat à l'Energie Atomique, INSERM, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble 38000, France (S.B., M.T.).
Pascaline AuroyCommissariat à l'Energie Atomique, Centre National de la Recherche Scientifique Aix Marseille Université, Unité Mixte de Recherche 7265, Institut de Biosciences et Biotechnologies, Cadarache 13108, France (H.D.G., S.C., B.L., Y.L., P.A., H.J., F.B., G.P., Y.L.-B.);Faculty of Agriculture and the Environment, University of Sydney, Sydney, New South Wales 2006, Australia (H.D.G., B.J.); andCommissariat à l'Energie Atomique, INSERM, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble 38000, France (S.B., M.T.).
Hélène JavotCommissariat à l'Energie Atomique, Centre National de la Recherche Scientifique Aix Marseille Université, Unité Mixte de Recherche 7265, Institut de Biosciences et Biotechnologies, Cadarache 13108, France (H.D.G., S.C., B.L., Y.L., P.A., H.J., F.B., G.P., Y.L.-B.);Faculty of Agriculture and the Environment, University of Sydney, Sydney, New South Wales 2006, Australia (H.D.G., B.J.); andCommissariat à l'Energie Atomique, INSERM, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble 38000, France (S.B., M.T.).ORCID 0000-0002-4577-2747
Marianne TardifCommissariat à l'Energie Atomique, Centre National de la Recherche Scientifique Aix Marseille Université, Unité Mixte de Recherche 7265, Institut de Biosciences et Biotechnologies, Cadarache 13108, France (H.D.G., S.C., B.L., Y.L., P.A., H.J., F.B., G.P., Y.L.-B.);Faculty of Agriculture and the Environment, University of Sydney, Sydney, New South Wales 2006, Australia (H.D.G., B.J.); andCommissariat à l'Energie Atomique, INSERM, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble 38000, France (S.B., M.T.).
Brian JonesCommissariat à l'Energie Atomique, Centre National de la Recherche Scientifique Aix Marseille Université, Unité Mixte de Recherche 7265, Institut de Biosciences et Biotechnologies, Cadarache 13108, France (H.D.G., S.C., B.L., Y.L., P.A., H.J., F.B., G.P., Y.L.-B.);Faculty of Agriculture and the Environment, University of Sydney, Sydney, New South Wales 2006, Australia (H.D.G., B.J.); andCommissariat à l'Energie Atomique, INSERM, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble 38000, France (S.B., M.T.).
Fred BeissonCommissariat à l'Energie Atomique, Centre National de la Recherche Scientifique Aix Marseille Université, Unité Mixte de Recherche 7265, Institut de Biosciences et Biotechnologies, Cadarache 13108, France (H.D.G., S.C., B.L., Y.L., P.A., H.J., F.B., G.P., Y.L.-B.);Faculty of Agriculture and the Environment, University of Sydney, Sydney, New South Wales 2006, Australia (H.D.G., B.J.); andCommissariat à l'Energie Atomique, INSERM, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble 38000, France (S.B., M.T.).ORCID 0000-0001-9995-7387
Gilles PeltierCommissariat à l'Energie Atomique, Centre National de la Recherche Scientifique Aix Marseille Université, Unité Mixte de Recherche 7265, Institut de Biosciences et Biotechnologies, Cadarache 13108, France (H.D.G., S.C., B.L., Y.L., P.A., H.J., F.B., G.P., Y.L.-B.);Faculty of Agriculture and the Environment, University of Sydney, Sydney, New South Wales 2006, Australia (H.D.G., B.J.); andCommissariat à l'Energie Atomique, INSERM, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble 38000, France (S.B., M.T.).
Yonghua Li-BeissonCommissariat à l'Energie Atomique, Centre National de la Recherche Scientifique Aix Marseille Université, Unité Mixte de Recherche 7265, Institut de Biosciences et Biotechnologies, Cadarache 13108, France (H.D.G., S.C., B.L., Y.L., P.A., H.J., F.B., G.P., Y.L.-B.);Faculty of Agriculture and the Environment, University of Sydney, Sydney, New South Wales 2006, Australia (H.D.G., B.J.); andCommissariat à l'Energie Atomique, INSERM, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble 38000, France (S.B., M.T.) yonghua.li@cea.fr.ORCID 0000-0003-1064-1816
Inserm · FRCentre National de la Recherche Scientifique · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enriching algal biomass in energy density is an important goal in algal biotechnology. Nitrogen (N) starvation is considered the most potent trigger of oil accumulation in microalgae and has been thoroughly investigated. However, N starvation causes the slow down and eventually the arrest of biomass growth. In this study, we show that exposing a Chlamydomonas reinhardtii culture to saturating light (SL) under a nonlimiting CO2 concentration in turbidostatic photobioreactors induces a sustained accumulation of lipid droplets (LDs) without compromising growth, which results in much higher oil productivity than N starvation. We also show that the polar membrane lipid fraction of SL-induced LDs is rich in plastidial lipids (approximately 70%), in contrast to N starvation-induced LDs, which contain approximately 60% lipids of endoplasmic reticulum origin. Proteomic analysis of LDs isolated from SL-exposed cells identified more than 200 proteins, including known proteins of lipid metabolism, as well as 74 proteins uniquely present in SL-induced LDs. LDs induced by SL and N depletion thus differ in protein and lipid contents. Taken together, lipidomic and proteomic data thus show that a large part of the sustained oil accumulation occurring under SL is likely due to the formation of plastidial LDs. We discuss our data in relation to the different metabolic routes used by microalgae to accumulate oil reserves depending on cultivation conditions. Finally, we propose a model in which oil accumulation is governed by an imbalance between photosynthesis and growth, which can be achieved by impairing growth or by boosting photosynthetic carbon fixation, with the latter resulting in higher oil productivity.

Indexed as

Lipid MetabolismProteomicsBiomassChlamydomonas reinhardtiiLightLipid DropletsMicroalgaeNitrogenPhotosynthesisNitrogen

Identifiers

PMID27297678
PMCPMC4972293
OpenAlexW2427832593

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.