ArticleBMC microbiology2016
16S-ARDRA and MALDI-TOF mass spectrometry as tools for identification of Lactobacillus bacteria isolated from poultry.
Article in BMC microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed.
- Decoding anaerobes: a comprehensive evaluation of MALDI-TOF Sirius and VITEK MS PRIME in clinical settings.Microbiology spectrum · 2025Article
- Susceptibility ofLife (Basel, Switzerland) · 2025Article
- Diversity of Fecal Indicator Enterococci among Different Hosts: Importance to Water Contamination Source Tracking.Microorganisms · 2023Article
- Research Progresses on the Function and Detection Methods of Insect Gut Microbes.Microorganisms · 2023Review
- Selection and evaluation of lactic acid bacteria from chicken feces in Thailand as potential probiotics.PeerJ · 2023Article
- Characterization of bacterial diversity between two coastal regions with heterogeneous soil texture.Scientific reports · 2022Article
- Alarming Antibiotic Resistance of Lactobacilli Isolated from Probiotic Preparations and Dietary Supplements.Antibiotics (Basel, Switzerland) · 2022Article
- Innovative Use of Olive, Winery and Cheese Waste By-Products as Functional Ingredients in Broiler Nutrition.Veterinary sciences · 2022Article
- Isolation and probiotic potential of lactic acid bacteria from swine feces for feed additive composition.Archives of microbiology · 2021Article
- Therapeutic and Prophylactic Effect of the Experimental Bacteriophage Treatment to Control Diarrhea Caused byACS infectious diseases · 2021Article
- Biodiversity ofAnimals : an open access journal from MDPI · 2021Article
- Evaluation of the Autof MS1000 mass spectrometer in the identification of clinical isolates.BMC microbiology · 2020Article
- Influence of High Hydrostatic Pressure on the Identification ofMicroorganisms · 2020Article
- Molecular Routes to Specific Identification of the Lactobacillus Casei Group at the Species, Subspecies and Strain Level.International journal of molecular sciences · 2020Article
- Susceptibility of chicken Lactobacillus bacteria to coccidiostats.The Journal of veterinary medical science · 2020Article
- Identification and molecular characterization of Azotobacter chroococcum and Azotobacter salinestris using ARDRA, REP, ERIC, and BOX.Molecular biology reports · 2020Article
- Identification, Classification and Screening for γ-Amino-butyric Acid Production in Lactic Acid Bacteria from Cambodian Fermented Foods.Biomolecules · 2019Article
- Article
- Identification and antibiotic susceptibility of lactobacilli isolated from turkeys.BMC microbiology · 2018Article
- Probiotic potential of Lactobacillus isolates of chicken origin with anti-Campylobacter activity.The Journal of veterinary medical science · 2018Article
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Abstract
backgroundThe objective of our study is to evaluate the potential use of Amplified 16S Ribosomal DNA Restriction Analysis (16S-ARDRA) and MALDI-TOF mass spectrometry (MS) as methods for species identification of Lactobacillus strains in poultry.
resultsA total of 80 Lactobacillus strains isolated from the cloaca of chicken, geese and turkeys were identified to the species level by MALDI-TOF MS (on-plate extraction method) and 16S-ARDRA. The two techniques produced comparable classification results, some of which were additionally confirmed by sequencing of 16S rDNA. MALDI-TOF MS enabled rapid species identification but produced more than one reliable identification result for 16.25 % of examined strains (mainly of the species L. johnsonii). For 30 % of isolates intermediate log(scores) of 1.70-1.99 were obtained, indicating correct genus identification but only presumptive species identification. The 16S-ARDRA protocol was based on digestion of 16S rDNA with the restriction enzymes MseI, HinfI, MboI and AluI. This technique was able to distinguish 17 of the 19 Lactobacillus reference species tested and enabled identification of all 80 wild isolates. L. salivarius dominated among the 15 recognized species, followed by L. johnsonii and L. ingluviei.
conclusionsThe MALDI-TOF MS and 16S-ARDRA assays are valuable tools for the identification of avian lactobacilli to the species level. MALDI-TOF MS is a fast, simple and cost-effective technique, and despite generating a high percentage of results with a log(score) <2.00, the on-plate extraction method is characterized by high-performance. For samples for which Biotyper produces more than one reliable result, MALDI-TOF MS must be used in combination with genotypic techniques to achieve unambiguous results. 16S-ARDRA is simple, repetitive method with high power of discrimination, whose sole limitation is its inability to discriminate between species with very high 16S rDNA sequence homology, such as L. casei and L. zeae. The assays can be used for discrimination of Lactobacillus bacteria from different habitats.
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