ArticleScientific reports2016
Intracellular ROS mediates gas plasma-facilitated cellular transfection in 2D and 3D cultures.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Pre-clinical Safety Study of Cold Atmospheric Plasma (CAP) Produced by an Inbuilt CAP Device and ROS Mediated Apoptotic Activity in Human Skin Melanoma Cells.Current pharmaceutical design · 2026Article
- Subcytotoxic transepidermal delivery using low intensity cold atmospheric plasma.Scientific reports · 2025Article
- Synergistic effects of plasma-activated medium in combination with Baicalin against neuronal damage.Heliyon · 2024Article
- Site-selected in situ polymerization for living cell surface engineering.Nature communications · 2023Article
- Synthesis, Biophysical Properties, and Antitumor Activity of Antisense Oligonucleotides Conjugated with Anisamide.Pharmaceutics · 2023Article
- Plasma Promotes Fungal Cellulase Production by Regulating the Levels of Intracellular NO and CaInternational journal of molecular sciences · 2022Article
- Carrier-Free Cellular Transport of CRISPR/Cas9 Ribonucleoprotein for Genome Editing by Cold Atmospheric Plasma.Biology · 2021Article
- Impact of Reactive Oxygen and Nitrogen Species Produced by Plasma on Mdm2-p53 Complex.International journal of molecular sciences · 2021Article
- Delivery and expression of plasmid DNA into cells by a novel non-thermal plasma source.Bioelectrochemistry (Amsterdam, Netherlands) · 2021Article
- Article
- Aberrant Expressional Profiling of Small RNA by Cold Atmospheric Plasma Treatment in Human Chronic Myeloid Leukemia Cells.Frontiers in genetics · 2021Article
- Plasma enhance drug sensitivity to bortezomib by inhibition of cyp1a1 in myeloma cells.Translational cancer research · 2019Article
- Direct transfection of clonal organoids in Matrigel microbeads: a promising approach toward organoid-based genetic screens.Nucleic acids research · 2018Article
- Alteration of metabolite profiling by cold atmospheric plasma treatment in human myeloma cells.Cancer cell international · 2018Article
- Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells.Journal of visualized experiments : JoVE · 2017Article
- How Does Membrane Oxidation Affect Cell Delivery and Cell Killing?Trends in biotechnology · 2017Article
- Surface chemistry and germination improvement of Quinoa seeds subjected to plasma activation.Scientific reports · 2017Article
- Direct evidence for cell adhesion-mediated radioresistance (CAM-RR) on the level of individual integrin β1 clusters.Scientific reports · 2017Article
- Membrane Oxidation in Cell Delivery and Cell Killing Applications.ACS chemical biology · 2017Review
- Gas-liquid interfacial plasmas producing reactive species for cell membrane permeabilization.Journal of clinical biochemistry and nutrition · 2017Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study reports the potential of cold atmospheric plasma (CAP) as a versatile tool for delivering oligonucleotides into mammalian cells. Compared to lipofection and electroporation methods, plasma transfection showed a better uptake efficiency and less cell death in the transfection of oligonucleotides. We demonstrated that the level of extracellular aqueous reactive oxygen species (ROS) produced by gas plasma is correlated with the uptake efficiency and that this is achieved through an increase of intracellular ROS levels and the resulting increase in cell membrane permeability. This finding was supported by the use of ROS scavengers, which reduced CAP-based uptake efficiency. In addition, we found that cold atmospheric plasma could transfer oligonucleotides such as siRNA and miRNA into cells even in 3D cultures, thus suggesting the potential for unique applications of CAP beyond those provided by standard transfection techniques. Together, our results suggest that cold plasma might provide an efficient technique for the delivery of siRNA and miRNA in 2D and 3D culture models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.