ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2016
Efficient generation of transgenic reporter strains and analysis of expression patterns in Caenorhabditis elegans using library MosSCI.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 33 citations in OpenAlex.
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- Benzimidazoles cause lethality by inhibiting the function of Caenorhabditis elegans neuronal beta-tubulin.International journal for parasitology. Drugs and drug resistance · 2022Article
- The ERM-1 membrane-binding domain directs erm-1 mRNA localization to the plasma membrane in the C. elegans embryo.Development (Cambridge, England) · 2022Article
- Modular safe-harbor transgene insertion for targeted single-copy and extrachromosomal array integration in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2022Article
- Surveying Low-Cost Methods to Measure Lifespan and Healthspan in Caenorhabditis elegans.Journal of visualized experiments : JoVE · 2022Article
- The role of RNA-binding proteins in orchestrating germline development inFrontiers in cell and developmental biology · 2022Review
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- The endogenous mex-3 3´UTR is required for germline repression and contributes to optimal fecundity in C. elegans.PLoS genetics · 2021Article
- An improved in vivo tethering assay with single molecule FISH reveals that a nematode Nanos enhances reporter expression and mRNA stability.RNA (New York, N.Y.) · 2021Article
- Tissue-Specific Split sfGFP System for Streamlined Expression of GFP Tagged Proteins in theG3 (Bethesda, Md.) · 2019Article
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7 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundIn C. elegans, germline development and early embryogenesis rely on posttranscriptional regulation of maternally transcribed mRNAs. In many cases, the 3' untranslated region (UTR) is sufficient to govern the expression patterns of these transcripts. Several RNA-binding proteins are required to regulate maternal mRNAs through the 3'UTR. Despite intensive efforts to map RNA-binding protein-mRNA interactions in vivo, the biological impact of most binding events remains unknown. Reporter studies using single copy integrated transgenes are essential to evaluate the functional consequences of interactions between RNA-binding proteins and their associated mRNAs.
resultsIn this report, we present an efficient method of generating reporter strains with improved throughput by using a library variant of MosSCI transgenesis. Furthermore, using RNA interference, we identify the suite of RNA-binding proteins that control the expression pattern of five different maternal mRNAs.
conclusionsThe results provide a generalizable and efficient strategy to assess the functional relevance of protein-RNA interactions in vivo, and reveal new regulatory connections between key RNA-binding proteins and their maternal mRNA targets. Developmental Dynamics 245:925-936, 2016. © 2016 Wiley Periodicals, Inc.
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