ArticleCell reports2016
Protein Abundance Control by Non-coding Antisense Transcription.
Article in Cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 59 citations in OpenAlex.
- The hidden power of antisense long non-coding RNAs: a dive into a novel regulatory layer mediated by double-stranded RNA formation.RNA biology · 2025Review
- Identification and Functions of lncRNAs in Fungi.Non-coding RNA · 2025Review
- Functional analysis and transcriptional profiling of non-coding RNAs in yeast.The Biochemical journal · 2025Review
- Pervasive formation of double-stranded RNAs by overlapping sense/antisense transcripts in budding yeast mitosis and meiosis.RNA (New York, N.Y.) · 2025Article
- Article
- Post-transcriptional regulation shapes the transcriptome of quiescent budding yeast.Nucleic acids research · 2024Article
- Differential expression patterns of long noncoding RNAs in a pleiomorphic diatom and relation to hyposalinity.Scientific reports · 2023Article
- The RNA-binding protein RBP33 dampens non-productive transcription in trypanosomes.Nucleic acids research · 2022Article
- Antisense non-coding transcription represses the PHO5 model gene at the level of promoter chromatin structure.PLoS genetics · 2022Article
- Article
- Modulated termination of non-coding transcription partakes in the regulation of gene expression.Nucleic acids research · 2022Article
- Article
- Uncovering de novo gene birth in yeast using deep transcriptomics.Nature communications · 2021Article
- Epigenomic Reprogramming as a Driver of Malignant Glioma.Cancer cell · 2020Review
- Unique and Shared Roles for Histone H3K36 Methylation States in Transcription Regulation Functions.Cell reports · 2020Article
- Advances in RNAi-Assisted Strain Engineering inFrontiers in bioengineering and biotechnology · 2020Review
- Histone H2B Ubiquitylation Regulates Histone Gene Expression by Suppressing Antisense Transcription in Fission Yeast.Genetics · 2019Article
- A current view on long noncoding RNAs in yeast and filamentous fungi.Applied microbiology and biotechnology · 2018Review
- Genome-wide C-SWAT library for high-throughput yeast genome tagging.Nature methods · 2018Article
- Antisense transcriptional interference mediates condition-specific gene repression in budding yeast.Nucleic acids research · 2018Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stable unannotated transcripts (SUTs), some of which overlap protein-coding genes in antisense direction, are a class of non-coding RNAs. While case studies have reported important regulatory roles for several of such RNAs, their general impact on protein abundance regulation of the overlapping gene is not known. To test this, we employed seamless gene manipulation to repress antisense SUTs of 162 yeast genes by using a unidirectional transcriptional terminator and a GFP tag. We found that the mere presence of antisense SUTs was not sufficient to influence protein abundance, that observed effects of antisense SUTs correlated with sense transcript start site overlap, and that the effects were generally weak and led to reduced protein levels. Antisense regulated genes showed increased H3K4 di- and trimethylation and had slightly lower than expected noise levels. Our results suggest that the functionality of antisense RNAs has gene and condition-specific components.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.