Evidence map›Paper›PMID 27292569›Full record

ArticleScientific reports2016

N-cadherin is Key to Expression of the Nucleus Pulposus Cell Phenotype under Selective Substrate Culture Conditions.

Priscilla Y Hwang, Liufang Jing, Jun Chen, Foon-Lian Lim, Ruhang Tang, Hyowon Choi, Kenneth M Cheung, Makarand V Risbud, Charles A Gersbach, Farshid Guilak and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Priscilla Y HwangDepartment of Biomedical Engineering, Duke University, Durham NC 27713, USA.
Liufang JingDepartment of Biomedical Engineering, Duke University, Durham NC 27713, USA.
Jun ChenDepartment of Orthopaedic Surgery, Duke University Medical Center, Durham NC 27713, USA.
Foon-Lian LimDepartments of Orthopaedics &Traumatology, The University of Hong Kong, Hong Kong SAR, China.
Ruhang TangDepartment of Orthopaedic Surgery, Washington University in St. Louis, St. Louis Missouri 63110, USA.
Hyowon ChoiDepartment of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia PA, USA.
Kenneth M CheungDepartments of Orthopaedics &Traumatology, The University of Hong Kong, Hong Kong SAR, China.
Makarand V RisbudDepartment of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia PA, USA.
Charles A GersbachDepartment of Biomedical Engineering, Duke University, Durham NC 27713, USA.
Farshid GuilakDepartment of Biomedical Engineering, Duke University, Durham NC 27713, USA.
Victor Y LeungDepartments of Orthopaedics &Traumatology, The University of Hong Kong, Hong Kong SAR, China.
Lori A SettonDepartment of Biomedical Engineering, Duke University, Durham NC 27713, USA.

Funding

MECHANICAL STIMULATION OF IVD CELLSR01AR047442 · NIAMS · WASHINGTON UNIVERSITY · PI SETTON, LORI A. · 2001 to 2015
$4.6M
Engineering Microenvironments for the Nucleus Pulposus CellR01AR069588 · NIAMS · WASHINGTON UNIVERSITY · PI SETTON, LORI A. · 2016 to 2019
$1.2M
Developing Immature Nucleus Pulposus Cells for Regenerative TherapyR01AR057410 · NIAMS · DUKE UNIVERSITY · PI CHEN, JUN · 2009 to 2013
$1.2M
NIAMS NIH HHS R01 AR047442NIAMS NIH HHS R01 AR057410NIAMS NIH HHS R01 AR069588
6 · The paper itself

Abstract

Nucleus pulposus (NP) cells of the intervertebral disc are essential for synthesizing extracellular matrix that contributes to disc health and mechanical function. NP cells have a unique morphology and molecular expression pattern derived from their notochordal origin, and reside in N-cadherin (CDH2) positive cell clusters in vivo. With disc degeneration, NP cells undergo morphologic and phenotypic changes including loss of CDH2 expression and ability to form cell clusters. Here, we investigate the role of CDH2 positive cell clusters in preserving healthy, biosynthetically active NP cells. Using a laminin-functionalized hydrogel system designed to mimic features of the native NP microenvironment, we demonstrate NP cell phenotype and morphology is preserved only when NP cells form CDH2 positive cell clusters. Knockdown (CRISPRi) or blocking CDH2 expression in vitro and in vivo results in loss of a healthy NP cell. Findings also reveal that degenerate human NP cells that are CDH2 negative can be promoted to re-express CDH2 and healthy, juvenile NP matrix synthesis patterns by promoting cell clustering for controlled microenvironment conditions. This work also identifies CDH2 interactions with β-catenin-regulated signaling as one mechanism by which CDH2-mediated cell interactions can control NP cell phenotype and biosynthesis towards maintenance of healthy intervertebral disc tissues.

Indexed as

AdolescentAdultAgedAnimalsAntigens, CDbeta CateninCadherinsCell CommunicationCell Culture TechniquesCells, CulturedChildGene Knockdown TechniquesHumansMiddle AgedNucleus PulposusPhenotypeAntigens, CDbeta CateninCadherinsCDH2 protein, human

Identifiers

PMID27292569
PMCPMC4904275

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.