ArticleNeoplasia (New York, N.Y.)2016
Early Detection of Treatment-Induced Mitotic Arrest Using Temporal Diffusion Magnetic Resonance Spectroscopy.
Article in Neoplasia (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- Risk Stratification Prediction of Endometrial Cancer Using Microstructural Mapping Based on Time-Dependent Diffusion MRI.Cancer science · 2025Article
- Microstructural diffusion MRI for differentiation of breast tumors and prediction of prognostic factors in breast cancer.Frontiers in oncology · 2025Article
- Detection of Treatment Response in Triple-Negative Breast Tumors to Paclitaxel Using MRI Cell Size Imaging.Journal of magnetic resonance imaging : JMRI · 2024Article
- Molecular Imaging with Aquaporin-Based Reporter Genes: Quantitative Considerations from Monte Carlo Diffusion Simulations.ACS synthetic biology · 2023Article
- Molecular imaging with aquaporin-based reporter genes: quantitative considerations from Monte Carlo diffusion simulations.bioRxiv : the preprint server for biology · 2023Article
- Improving MR cell size imaging by inclusion of transcytolemmal water exchange.NMR in biomedicine · 2022Article
- Towards differentiation of brain tumor from radiation necrosis using multi-parametric MRI: Preliminary results at 4.7 T using rodent models.Magnetic resonance imaging · 2022Article
- MR cell size imaging with temporal diffusion spectroscopy.Magnetic resonance imaging · 2021Review
- MRI-cytometry: Mapping nonparametric cell size distributions using diffusion MRI.Magnetic resonance in medicine · 2021Article
- Probing neural tissues at small scales: Recent progress of oscillating gradient spin echo (OGSE) neuroimaging in humans.Journal of neuroscience methods · 2021Article
- Mapping hepatocyte size in vivo using temporal diffusion spectroscopy MRI.Magnetic resonance in medicine · 2020Article
- MRI of tumor T cell infiltration in response to checkpoint inhibitor therapy.Journal for immunotherapy of cancer · 2020Article
- Magnetic resonance imaging of mean cell size in human breast tumors.Magnetic resonance in medicine · 2020Article
- Microstructure Modeling of High b-Value Diffusion-Weighted Images in Glioblastoma.Tomography (Ann Arbor, Mich.) · 2020Article
- In vivo magnetic resonance imaging of treatment-induced apoptosis.Scientific reports · 2019Article
- Oscillating-gradient diffusion magnetic resonance imaging detects acute subcellular structural changes in the mouse forebrain after neonatal hypoxia-ischemia.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2019Article
- Oscillating gradient diffusion kurtosis imaging of normal and injured mouse brains.NMR in biomedicine · 2018Article
- Modeling Cancer Cell Growth DynamicsFrontiers in oncology · 2017Article
- Quantitative temporal diffusion spectroscopy as an early imaging biomarker of radiation therapeutic response in gliomas: A preclinical proof of concept.Advances in radiation oncologyArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
purposeA novel quantitative magnetic resonance imaging (MRI) method, namely, temporal diffusion spectroscopy (TDS), was used to detect the response of tumor cells (notably, mitotic arrest) to a specific antimitotic treatment (Nab-paclitaxel) in culture and human ovarian xenografts and evaluated as an early imaging biomarker of tumor responsiveness.
methodsTDS measures a series of apparent diffusion coefficients (ADCs) of tissue water over a range of effective diffusion times, which may correspond to diffusion distances ranging from subcellular to cellular levels (~3-20 μm). By fitting the measured ADC data to a tissue model, parameters reflecting structural properties such as restriction size in solid tumors can be extracted. Two types of human ovarian cell lines (OVCAR-8 as a responder to Nab-paclitaxel and NCI/ADR-RES as a resistant type) were treated with either vehicle (PBS) or Nab-paclitaxel, and treatment responses of both in vitro and in vivo cases were investigated using TDS.
resultsAcute cell size increases induced by Nab-paclitaxel in responding tumors were confirmed by flow cytometry and light microscopy in cell culture. Nab-paclitaxel-induced mitotic arrest in treated tumors/cells was quantified histologically by measuring the mitotic index in vivo using a mitosis-specific marker (anti-phosphohistone H3). Changes in the fitted restriction size, one of the parameters obtained from TDS, were able to detect and quantify increases in tumor cell sizes. All the MR results had a high degree of consistency with other flow, microscopy, and histological data. Moreover, with an appropriate analysis, the Nab-paclitaxel-responsive tumors in vivo could be easily distinguished from all the other vehicle-treated and Nab-paclitaxel-resistant tumors.
conclusionTDS detects increases in cell sizes associated with antimitotic-therapy-induced mitotic arrest in solid tumors in vivo which occur before changes in tissue cellularity or conventional diffusion MRI metrics. By quantifying changes in cell size, TDS has the potential to improve the specificity of MRI methods in the evaluation of therapeutic response and enable a mechanistic understanding of therapy-induced changes in tumors.
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