ArticlePlanta2016
New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents.
Article in Planta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 45 citations in OpenAlex.
- Review
- Physical Mapping of a Powdery Mildew Resistance Gene in Chromosome 6St from Wheat-Plants (Basel, Switzerland) · 2026Article
- Replacement of chromosome 3D with Thinopyrum chromosome 3St led to increased drought tolerance during the flowering stage in wheat.Plant cell reports · 2025Article
- The launch of satellite: DNA repeats as a cytogenetic tool in discovering the chromosomal universe of wild Triticeae.Chromosoma · 2023Review
- Development and cytological characterization of wheat-Frontiers in plant science · 2023Article
- The improvement of agronomic performances in the cold weather conditions for perennial wheatgrass by crossingFrontiers in plant science · 2023Article
- Molecular and Cytogenetic Identification of Wheat-Plants (Basel, Switzerland) · 2022Article
- Development and application of universal ND-FISH probes for detecting P-genome chromosomes based onMolecular breeding : new strategies in plant improvement · 2022Article
- Cytogenetic identification and molecular marker development of a novel wheat-Thinopyrum ponticum translocation line with powdery mildew resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat-Thinopyrum intermedium and wheat-Thinopyrum ponticum substitution lines.BMC plant biology · 2022Article
- Article
- A universal karyotypic system for hexaploid and diploid Avena species brings oat cytogenetics into the genomics era.BMC plant biology · 2021Article
- Development of Sequence-Tagged Site Marker Set for Identification of J, JFrontiers in plant science · 2021Article
- Identification of a Wheat-Frontiers in plant science · 2021Article
- Development of SpecificInternational journal of molecular sciences · 2020Article
- Quantitative Trait Locus (QTLs) Mapping for Quality Traits of Wheat Based on High Density Genetic Map Combined With Bulked Segregant Analysis RNA-seq (BSR-Seq) Indicates That theFrontiers in plant science · 2020Article
- Cytogenetic Analysis and Molecular Marker Development for a New Wheat-Frontiers in plant science · 2020Article
- Development and validation of an exome-based SNP marker set for identification of the St, JTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- New ND-FISH-Positive Oligo Probes for IdentifyingInternational journal of molecular sciences · 2019Article
- Molecular-cytogenetic analysis of diploid wheatgrassComparative cytogenetics · 2019Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionNew molecular markers were developed for targeting Thinopyrum intermedium 1St#2 chromosome, and novel FISH probe representing the terminal repeats was produced for identification of Thinopyrum chromosomes. Thinopyrum intermedium has been used as a valuable resource for improving the disease resistance and yield potential of wheat. A wheat-Th. intermedium ssp. trichophorum chromosome 1St#2 substitution and translocation has displayed superior grain protein and wet gluten content. With the aim to develop a number of chromosome 1St#2 specific molecular and cytogenetic markers, a high throughput, low-cost specific-locus amplified fragment sequencing (SLAF-seq) technology was used to compare the sequences between a wheat-Thinopyrum 1St#2 (1D) substitution and the related species Pseudoroegneria spicata (St genome, 2n = 14). A total of 5142 polymorphic fragments were analyzed and 359 different SLAF markers for 1St#2 were predicted. Thirty-seven specific molecular markers were validated by PCR from 50 randomly selected SLAFs. Meanwhile, the distribution of transposable elements (TEs) at the family level between wheat and St genomes was compared using the SLAFs. A new oligo-nucleotide probe named Oligo-pSt122 from high SLAF reads was produced for fluorescence in situ hybridization (FISH), and was observed to hybridize to the terminal region of 1St#L and also onto the terminal heterochromatic region of Th. intermedium genomes. The genome-wide markers and repetitive based probe Oligo-pSt122 will be valuable for identifying Thinopyrum chromosome segments in wheat backgrounds.
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