Evidence map›Paper›PMID 27289464›Full record

ArticleAnalytical and bioanalytical chemistry2016

On-column entrapment of alpha1-acid glycoprotein for studies of drug-protein binding by high-performance affinity chromatography.

Jeanethe Anguizola, Cong Bi, Michelle Koke, Abby Jackson, David S Hage

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Article
  3. Characterization of binding by sulfonylureas with normal or modified human serum albumin using affinity microcolumns prepared by entrapment.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2023
    Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Optimization of protein entrapment in affinity microcolumns using hydrazide-activated silica and glycogen as a capping agent.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2019
    Article
  10. Review
  11. Review
  12. Review
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jeanethe AnguizolaChemistry Department, University of Nebraska, 704 Hamilton Hall, Lincoln, NE, 68588, USA.
Cong BiChemistry Department, University of Nebraska, 704 Hamilton Hall, Lincoln, NE, 68588, USA.
Michelle KokeChemistry Department, University of Nebraska, 704 Hamilton Hall, Lincoln, NE, 68588, USA.
Abby JacksonChemistry Department, University of Nebraska, 704 Hamilton Hall, Lincoln, NE, 68588, USA.
David S HageChemistry Department, University of Nebraska, 704 Hamilton Hall, Lincoln, NE, 68588, USA. dhage1@unl.edu.
University of Nebraska–Lincoln · US

Funding

CHROMATOGRAPHIC AUTOMATION OF IMMUNOASSAYSR01GM044931 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI HAGE, DAVID S · 1998 to 2014
$2.7M
CHROMATOGRAPHIC STUDIES OF FUNCTIONAL PROTEOMICSR01DK069629 · NIDDK · UNIVERSITY OF NEBRASKA LINCOLN · PI HAGE, DAVID S · 2006 to 2017
$1.8M
NIDDK NIH HHS R01 DK069629NIGMS NIH HHS R01 GM044931
6 · The paper itself

Abstract

An on-column approach for protein entrapment was developed to immobilize alpha1-acid glycoprotein (AGP) for drug-protein binding studies based on high-performance affinity chromatography. Soluble AGP was physically entrapped by using microcolumns that contained hydrazide-activated porous silica and by employing mildly oxidized glycogen as a capping agent. Three on-column entrapment methods were evaluated and compared to a previous slurry-based entrapment method. The final selected method was used to prepare 1.0 cm × 2.1 mm I.D. affinity microcolumns that contained up to 21 (±4) μg AGP and that could be used over the course of more than 150 sample applications. Frontal analysis and zonal elution studies were performed on these affinity microcolumns to examine the binding of various drugs with the entrapped AGP. Site-selective competition studies were also conducted for these drugs. The results showed good agreement with previous observations for these drug-protein systems and with binding constants that have been reported in the literature. The entrapment method developed in this study should be useful for future work in the area of personalized medicine and in the high-throughput screening of drug interactions with AGP or other proteins. Graphical abstract On-column protein entrapment using a hydrazide-activated support and oxidized glycogen as a capping agent.

Indexed as

Antihypertensive AgentsChromatography, AffinityChromatography, High Pressure LiquidEquipment DesignHumansOrosomucoidPharmaceutical PreparationsPropranololProtein BindingWarfarinAntihypertensive AgentsOrosomucoidPharmaceutical PreparationsPropranololWarfarinAffinity microcolumnAlpha1-acid glycoproteinDrug-protein bindingEntrapmentHigh-performance affinity chromatographyImmobilization method

Identifiers

PMID27289464
PMCPMC4958572
OpenAlexW2417620732

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.