ArticleActa tropica2016
Optimization of loop-mediated isothermal amplification (LAMP) assays for the detection of Leishmania DNA in human blood samples.
Article in Acta tropica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- Diagnosis of visceral and cutaneous leishmaniasis using loop-mediated isothermal amplification (LAMP) protocols: a systematic review and meta-analysis.Parasites & vectors · 2022Pooled it
- LAMP-coupled CRISPR-Cas12a assays: A promising new tool for molecular diagnosis of leishmaniasis.PLoS neglected tropical diseases · 2026Article
- Mpox disease, diagnosis, and point of care platforms.Bioengineering & translational medicine · 2025Review
- Compact highly sensitive photothermal RT-LAMP chip for simultaneous multidisease detection.Science advances · 2024Article
- The Use of Dried Matrix Spots as an Alternative Sampling Technique for Monitoring Neglected Tropical Diseases.Pathogens (Basel, Switzerland) · 2024Review
- An overview of molecular markers for identification of non-human fecal pollution sources.Frontiers in microbiology · 2023Review
- A Novel Multiplex LAMP Assay for the Rapid and Accurate Diagnosis of Visceral Leishmaniasis Caused byJournal of tropical medicine · 2023Article
- Isothermal Nucleic Acid Amplification to Detect Infection Caused by Parasites of the Trypanosomatidae Family: A Literature Review and Opinion on the Laboratory to Field Applicability.International journal of molecular sciences · 2022Review
- Loop-Mediated Isothermal Amplification for Detection of Plant Pathogens in Wheat (Frontiers in plant science · 2022Review
- The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens.Plants (Basel, Switzerland) · 2021Review
- Clinical validation of optimised RT-LAMP for the diagnosis of SARS-CoV-2 infection.Scientific reports · 2021Article
- Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP.Nature communications · 2021Article
- Molecular Tracking of theFrontiers in cellular and infection microbiology · 2021Review
- A highly effective reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of SARS-CoV-2 infection.The Journal of infection · 2021Article
- Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis for Neglected Parasitic Infections.International journal of molecular sciences · 2020Review
- Loop-mediated isothermal amplification (LAMP): An advanced molecular point-of-care technique for the detection of Leishmania infection.PLoS neglected tropical diseases · 2019Review
- Mathematical model to reduce loop mediated isothermal amplification (LAMP) false-positive diagnosis.Electrophoresis · 2019Article
- Development and Assessment ofThe American journal of tropical medicine and hygiene · 2019Article
- Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection ofBioscience reports · 2019Article
- Multiplex loop-mediated isothermal amplification (multi-LAMP) assay for rapid detection ofInfection and drug resistance · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Visceral leishmaniasis (VL), one of the most important neglected tropical diseases, is caused by Leishmania donovani eukaryotic protozoan parasite of the genus Leishmania, the disease is prevalent mainly in the Indian sub-continent, East Africa and Brazil. VL can be diagnosed by PCR amplifying ITS1 and/or kDNA genes. The current study involved the optimization of Loop-mediated isothermal amplification (LAMP) for the detection of Leishmania DNA in human blood or tissue samples. Three LAMP systems were developed; in two of those the primers were designed based on shared regions of the ITS1 gene among different Leishmania species, while the primers for the third LAMP system were derived from a newly identified repeated region in the Leishmania genome. The LAMP tests were shown to be sufficiently sensitive to detect 0.1pg of DNA from most Leishmania species. The green nucleic acid stain SYTO16, was used here for the first time to allow real-time monitoring of LAMP amplification. The advantage of real time-LAMP using SYTO 16 over end-point LAMP product detection is discussed. The efficacy of the real time-LAMP tests for detecting Leishmania DNA in dried blood samples from volunteers living in endemic areas, was compared with that of qRT-kDNA PCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.