ArticleNucleic acids research2016
Endonucleolytic cleavages by RNase E generate the mature 3' termini of the three proline tRNAs in Escherichia coli.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Carrot and stick: how RNase R contributes to function and destruction of the translation machinery.RNA biology · 2025Review
- Structural basis of transfer RNA processing by bacterial minimal RNase P.Nature communications · 2025Article
- Unraveling the interplay between a small RNA and RNase E in bacteria.Nucleic acids research · 2024Article
- Interactors and effects of overexpressing YlxR/RnpM, a conserved RNA binding protein in cyanobacteria.RNA biology · 2024Article
- The role of the 5' sensing function of ribonuclease E in cyanobacteria.RNA biology · 2024Article
- The recognition of structured elements by a conserved groove distant from domains associated with catalysis is an essential determinant of RNase E.Nucleic acids research · 2023Article
- The C nucleotide at the mature 5' end of the Escherichia coli proline tRNAs is required for the RNase E cleavage specificity at the 3' terminus as well as functionality.Nucleic acids research · 2022Article
- Proline codon pair selection determines ribosome pausing strength and translation efficiency in bacteria.Communications biology · 2021Article
- Generation of pre-tRNAs from polycistronic operons is the essential function of RNase P in Escherichia coli.Nucleic acids research · 2020Article
- Characterization of the Methanomicrobial Archaeal RNase Zs for Processing the CCA-Containing tRNA Precursors.Frontiers in microbiology · 2020Article
- Polynucleotide phosphorylase promotes the stability and function of Hfq-binding sRNAs by degrading target mRNA-derived fragments.Nucleic acids research · 2019Article
- New Insights into the Relationship between tRNA Processing and Polyadenylation in Escherichia coli.Trends in genetics : TIG · 2019Review
- Analysis of post-transcriptional RNA metabolism in prokaryotes.Methods (San Diego, Calif.) · 2019Review
- Polynucleotide phosphorylase: Not merely an RNase but a pivotal post-transcriptional regulator.PLoS genetics · 2018Review
- Enzymes Involved in Posttranscriptional RNA Metabolism in Gram-Negative Bacteria.Microbiology spectrum · 2018Review
- The host-encoded RNase E endonuclease as the crRNA maturation enzyme in a CRISPR-Cas subtype III-Bv system.Nature microbiology · 2018Article
- TheJournal of bacteriology · 2017Article
- Article
- Repairing tRNA termini: News from the 3' end.RNA biology · 2016Article
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3 authors.
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Abstract
We demonstrate here for the first time that proline tRNA 3' end maturation in Escherichia coli employs a one-step endonucleolytic pathway that does not involve any of the six 3' → 5' exonucleases (RNase T, RNase PH, RNase D, RNase BN, RNase II and polynucleotide phosphorylase [PNPase]) to generate the mature CCA terminus. Rather, RNase E is primarily responsible for the endonucleolytic removal of the entire Rho-independent transcription terminator associated with the proK, proL and proM primary transcripts by cleaving immediately downstream of the CCA determinant. In the absence of RNase E, RNase G and RNase Z are weakly able to process the proK and proM transcripts, while PNPase and RNase P are utilized in the processing of proL The terminator fragment derived from the endonucleolytic cleavage of proL transcript is degraded through a PNPase-dependent pathway. It is not clear which enzymes degrade the proK and proM terminator fragments. Our data also suggest that the mature 5' nucleotide of the proline tRNAs may be responsible for the cleavage specificity of RNase E at the 3' terminus.
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