ArticleThe EMBO journal2016
Transcription rate and transcript length drive formation of chromosomal interaction domain boundaries.
Article in The EMBO journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed.
- Bacterial 3D genome architecture: organization, regulation, and synthetic biology applications.Genome biology · 2026Review
- Evolutionary constraints on RNA polymerase gene positioning in the genome of fast-growing bacteria.Nucleic acids research · 2026Article
- The biophysical properties of the bacterial nucleoid are dynamic, heterogeneous, and responsive to perturbations of cellular processes.bioRxiv : the preprint server for biology · 2026Article
- Article
- Principles of bacterial genome organization, a conformational point of view.Molecular microbiology · 2025Review
- Physical models of bacterial chromosomes.Molecular microbiology · 2025Review
- Spatial chromosome organization and adaptation of the radiation-resistant extremophile Deinococcus radiodurans.The Journal of biological chemistry · 2025Article
- Article
- Spatio-temporal organization of theEcoSal Plus · 2024Review
- Evolution and function of chromatin domains across the tree of life.Nature structural & molecular biology · 2024Review
- Article
- An extrinsic motor directs chromatin loop formation by cohesin.The EMBO journal · 2024Article
- Nucleosomes play a dual role in regulating transcription dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Bacterial chromatin proteins, transcription, and DNA topology: Inseparable partners in the control of gene expression.Molecular microbiology · 2024Review
- Spatial Chromosome Organization and Adaptation ofMicroorganisms · 2024Article
- Transcription factor shapes chromosomal conformation and regulates gene expression in bacterial adaptation.Nucleic acids research · 2024Article
- Combined Study of Gene Expression and Chromosome Three-Dimensional Structure in Escherichia coli During Growth Process.Current microbiology · 2024Article
- Transcription-induced domains form the elementary constraining building blocks of bacterial chromosomes.Nature structural & molecular biology · 2024Article
- Shedding Light on Bacterial Chromosome Structure: Exploring the Significance of 3C-Based Approaches.Methods in molecular biology (Clifton, N.J.) · 2024Review
- The environmentally-regulated interplay between local three-dimensional chromatin organisation and transcription of proVWX in E. coli.Nature communications · 2023Article
15 more citing papers are in PubMed but not listed here.
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2 authors.
Funding
Abstract
Chromosomes in all organisms are highly organized and divided into multiple chromosomal interaction domains, or topological domains. Regions of active, high transcription help establish and maintain domain boundaries, but precisely how this occurs remains unclear. Here, using fluorescence microscopy and chromosome conformation capture in conjunction with deep sequencing (Hi-C), we show that in Caulobacter crescentus, both transcription rate and transcript length, independent of concurrent translation, drive the formation of domain boundaries. We find that long, highly expressed genes do not form topological boundaries simply through the inhibition of supercoil diffusion. Instead, our results support a model in which long, active regions of transcription drive local decompaction of the chromosome, with these more open regions of the chromosome forming spatial gaps in vivo that diminish contacts between DNA in neighboring domains. These insights into the molecular forces responsible for domain formation in Caulobacter likely generalize to other bacteria and possibly eukaryotes.
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