ArticleBiophysical journal2016
Solution Asymmetry and Salt Expand Fluid-Fluid Coexistence Regions of Charged Membranes.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Biomimetic Lipid Raft: Domain Stability and Interaction with Physiologically Active Molecules.Advances in experimental medicine and biology · 2024Review
- Physical Concept to Explain the Regulation of Lipid Membrane Phase Separation under Isothermal Conditions.Life (Basel, Switzerland) · 2023Review
- Charged Lipids Influence Phase Separation in Cell-Sized Liposomes Containing Cholesterol or Ergosterol.Membranes · 2022Article
- A Review of Optical Imaging Technologies for Microfluidics.Micromachines · 2022Review
- Influence of Charge Lipid Head Group Structures on Electric Double Layer Properties.Journal of chemical theory and computation · 2022Article
- Observations of Membrane Domain Reorganization in Mechanically Compressed Artificial Cells.Chembiochem : a European journal of chemical biology · 2019Article
- Interaction of SNARE Mimetic Peptides with Lipid bilayers: Effects of Secondary Structure, Bilayer Composition and Lipid Anchoring.Scientific reports · 2019Article
- Poly(Ionic Liquid) Nanoparticles Selectively Disrupt Biomembranes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density.Communications biology · 2019Article
- Charged giant unilamellar vesicles prepared by electroformation exhibit nanotubes and transbilayer lipid asymmetry.Scientific reports · 2018Article
- Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy.Journal of visualized experiments : JoVE · 2017Article
- GM1 Softens POPC Membranes and Induces the Formation of Micron-Sized Domains.Biophysical journal · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liquid-liquid phase separation in giant unilamellar vesicles (GUVs) leads to the formation of intramembrane domains. To mimic charged biological membranes, we studied phase separation and domain formation in GUVs of ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol. The GUVs were exposed to solutions of sucrose and high-saline buffer. The phase diagram was determined using epifluorescence microscopy for vesicle populations with symmetric and asymmetric solution compositions across the membranes. Trans-membrane solution asymmetry was found to affect the membrane phase state. Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence. These observations were confirmed on single GUVs using microfluidics and confocal microscopy. Moreover, we found that the miscibility temperatures markedly increased for vesicles in the presence of symmetric and asymmetric salt solutions. Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
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