ArticleThe Plant cell2016
The Metabolite Pathway between Bundle Sheath and Mesophyll: Quantification of Plasmodesmata in Leaves of C3 and C4 Monocots.
Article in The Plant cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed.
- Improved mesophyll-bundle sheath connectivity is achieved via different mechanisms in CThe New phytologist · 2026Article
- Article
- Systems analysis of leaf anatomy and transcriptome using unsupervised machine learning provides insight on photosynthesis development and evolution in non-model grasses.Plant direct · 2026Article
- Cell-to-cell connectivity: a future target for crop improvement.Journal of experimental botany · 2026Review
- Microscopic probing into plasmodesmata (PD) and PD-derived intercellular gateways (PdIGs): Beyond morphology.Protoplasma · 2026Review
- WSF: A Transformer-Based Framework for Microphenotyping and Genetic Analyzing of Wheat Stomatal Traits.Plants (Basel, Switzerland) · 2025Article
- Bundle sheath cell-dependent chloroplast movement in mesophyll cells of CScientific reports · 2025Article
- Novel resources to investigate leaf plasmodesmata formation in CThe Plant journal : for cell and molecular biology · 2024Article
- Sugar sensing in C4 source leaves: a gap that needs to be filled.Journal of experimental botany · 2024Review
- Multiple Roles of Glycerate Kinase-From Photorespiration to Gluconeogenesis, CInternational journal of molecular sciences · 2024Review
- Plasmodesmal connectivity in CThe New phytologist · 2024Article
- A year at the forefront of plasmodesmal biology.Biology open · 2023Review
- Spatial expression patterns of genes encoding sugar sensors in leaves of C4 and C3 grasses.Annals of botany · 2023Article
- Nano-enabled agriculture: How do nanoparticles cross barriers in plants?Plant communications · 2022Review
- 13CO2 labeling kinetics in maize reveal impaired efficiency of C4 photosynthesis under low irradiance.Plant physiology · 2022Article
- The walnut shell network: 3D visualisation of symplastic and apoplastic transport routes in sclerenchyma tissue.Planta · 2022Article
- Using breeding and quantitative genetics to understand the C4 pathway.Journal of experimental botany · 2022Article
- Accelerated remodeling of the mesophyll-bundle sheath interface in the maize C4 cycle mutant leaves.Scientific reports · 2022Article
- Metabolic profiles in C3, C3-C4 intermediate, C4-like, and C4 species in the genus Flaveria.Journal of experimental botany · 2022Article
- Article
Corrections and comments
- Erratum issuedCORRECTION.2018
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
C4 photosynthesis is characterized by a CO2-concentrating mechanism between mesophyll (M) and bundle sheath (BS) cells of leaves. This generates high metabolic fluxes between these cells, through interconnecting plasmodesmata (PD). Quantification of these symplastic fluxes for modeling studies requires accurate quantification of PD, which has proven difficult using transmission electron microscopy. Our new quantitative technique combines scanning electron microscopy and 3D immunolocalization in intact leaf tissues to compare PD density on cell interfaces in leaves of C3 (rice [Oryza sativa] and wheat [Triticum aestivum]) and C4 (maize [Zea mays] and Setaria viridis) monocot species. Scanning electron microscopy quantification of PD density revealed that C4 species had approximately twice the number of PD per pitfield area compared with their C3 counterparts. 3D immunolocalization of callose at pitfields using confocal microscopy showed that pitfield area per M-BS interface area was 5 times greater in C4 species. Thus, the two C4 species had up to nine times more PD per M-BS interface area (S. viridis, 9.3 PD µm(-2); maize, 7.5 PD µm(-2); rice 1.0 PD µm(-2); wheat, 2.6 PD µm(-2)). Using these anatomical data and measured photosynthetic rates in these C4 species, we have now calculated symplastic C4 acid flux per PD across the M-BS interface. These quantitative data are essential for modeling studies and gene discovery strategies needed to introduce aspects of C4 photosynthesis to C3 crops.
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