ArticleDevelopment (Cambridge, England)2016
Pins is not required for spindle orientation in the Drosophila wing disc.
Article in Development (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Microtubule pushing and cortical pulling cooperate to orient and position the mitotic spindle.The EMBO journal · 2026Article
- Zebrafish otic vesicle and mouse epididymis as model systems for studying columnar epithelial cell division.bioRxiv : the preprint server for biology · 2025Article
- AGS3 antagonizes LGN to balance oriented cell divisions and cell fate choices in mammalian epidermis.eLife · 2023Article
- The Drosophila mitotic spindle orientation machinery requires activation, not just localization.EMBO reports · 2023Article
- Understanding the underlying mechanisms governing spindle orientation: How far are we from there?Journal of cellular and molecular medicine · 2022Review
- Minimal functional domains of the core polarity regulator Dlg.Biology open · 2022Article
- The wing imaginal disc.Genetics · 2022Article
- Forced into shape: Mechanical forces in Drosophila development and homeostasis.Seminars in cell & developmental biology · 2021Review
- Article
- Adherens junction remodelling during mitotic rounding of pseudostratified epithelial cells.EMBO reports · 2020Article
- Eph signaling in mitotic spindle orientation: what´s your angle here?Cell cycle (Georgetown, Tex.) · 2019Article
- Junctional tumor suppressors interact with 14-3-3 proteins to control planar spindle alignment.The Journal of cell biology · 2019Article
- Division orientation: disentangling shape and mechanical forces.Cell cycle (Georgetown, Tex.) · 2019Review
- Eph signaling controls mitotic spindle orientation and cell proliferation in neuroepithelial cells.The Journal of cell biology · 2019Article
- Oriented Cell Divisions Are Not Required for Drosophila Wing Shape.Current biology : CB · 2019Article
- Tissue tension and not interphase cell shape determines cell division orientation in theThe EMBO journal · 2019Article
- The asymmetric cell division machinery in the spiral-cleaving egg and embryo of the marine annelid Platynereis dumerilii.BMC developmental biology · 2017Article
- Akap350 Recruits Eb1 to The Spindle Poles, Ensuring Proper Spindle Orientation and Lumen Formation in 3d Epithelial Cell Cultures.Scientific reports · 2017Article
- Automated mitotic spindle tracking suggests a link between spindle dynamics, spindle orientation, and anaphase onset in epithelial cells.Molecular biology of the cell · 2017Article
- Epithelial spindle orientation diversities and uncertainties: recent developments and lingering questions.F1000Research · 2017Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
In animal cells, mitotic spindles are oriented by the dynein/dynactin motor complex, which exerts a pulling force on astral microtubules. Dynein/dynactin localization depends on Mud/NUMA, which is typically recruited to the cortex by Pins/LGN. In Drosophila neuroblasts, the Inscuteable/Baz/Par-6/aPKC complex recruits Pins apically to induce vertical spindle orientation, whereas in epithelial cells Dlg recruits Pins laterally to orient the spindle horizontally. Here we investigate division orientation in the Drosophila imaginal wing disc epithelium. Live imaging reveals that spindle angles vary widely during prometaphase and metaphase, and therefore do not reliably predict division orientation. This finding prompted us to re-examine mutants that have been reported to disrupt division orientation in this tissue. Loss of Mud misorients divisions, but Inscuteable expression and aPKC, dlg and pins mutants have no effect. Furthermore, Mud localizes to the apical-lateral cortex of the wing epithelium independently of both Pins and cell cycle stage. Thus, Pins is not required in the wing disc because there are parallel mechanisms for Mud localization and hence spindle orientation, making it a more robust system than in other epithelia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.