Evidence map›Paper›PMID 27287764›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2016

The use of multiplex PCR for the diagnosis of viral severe acute respiratory infection in children: a high rate of co-detection during the winter season.

A A El Kholy, N A Mostafa, A A Ali, M M S Soliman, S A El-Sherbini, R I Ismail, N El Basha, R I Magdy, N El Rifai, D H Hamed

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In one paragraph

Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.

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  19. Saffold virus, an emerging human cardiovirus.Reviews in medical virology · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

A A El KholyDepartment of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt.
N A MostafaDepartment of Pediatrics, Faculty of Medicine, Cairo University, Cairo, 11562, Egypt.
A A AliDepartment of Pediatrics, Faculty of Medicine, Cairo University, Cairo, 11562, Egypt. draliaaadel@yahoo.com.
M M S SolimanDepartment of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt.
S A El-SherbiniDepartment of Pediatrics, Faculty of Medicine, Cairo University, Cairo, 11562, Egypt.
R I IsmailDepartment of Pediatrics, Faculty of Medicine, Cairo University, Cairo, 11562, Egypt.
N El BashaDepartment of Pediatrics, Faculty of Medicine, Cairo University, Cairo, 11562, Egypt.
R I MagdyDepartment of Pediatrics, Faculty of Medicine, Cairo University, Cairo, 11562, Egypt.
N El RifaiDepartment of Pediatrics, Faculty of Medicine, Cairo University, Cairo, 11562, Egypt.
D H HamedDepartment of Pediatrics, Faculty of Medicine, Cairo University, Cairo, 11562, Egypt.
Cairo University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Respiratory tract infection is a major cause of hospitalization in children. Although most such infections are viral in origin, it is difficult to differentiate bacterial and viral infections, as the clinical symptoms are similar. Multiplex polymerase chain reaction (PCR) methods allow testing for multiple pathogens simultaneously and are, therefore, gaining interest. This prospective case-control study was conducted from October 2013 to February 2014. Nasopharyngeal (NP) and oropharyngeal (throat) swabs were obtained from children admitted with severe acute respiratory infection (SARI) at a tertiary hospital. A control group of 40 asymptomatic children was included. Testing for 16 viruses was done by real-time multiplex PCR. Multiplex PCR detected a viral pathogen in 159/177 (89.9 %) patients admitted with SARI. There was a high rate of co-infection (46.9 %). Dual detections were observed in 64 (36.2 %), triple detections in 17 (9.6 %), and quadruple detections in 2 (1.1 %) of 177 samples. Seventy-eight patients required intensive care unit (ICU) admission, of whom 28 (35.8 %) had co-infection with multiple viruses. AdV, HBoV, HRV, HEV, and HCoV-OC43 were also detected among asymptomatic children. This study confirms the high rate of detection of viral nucleic acids by multiplex PCR among hospitalized children admitted with SARI, as well as the high rate of co-detection of multiple viruses. AdV, HBoV, HRV, HEV, and HCoV-OC43 were also detected in asymptomatic children, resulting in challenges in clinical interpretation. Studies are required to provide quantitative conclusions that will facilitate clinical interpretation and application of the results in the clinical setting.

Indexed as

Case-Control StudiesChild, PreschoolCoinfectionFemaleHumansInfantMaleMultiplex Polymerase Chain ReactionNasopharynxOropharynxPrevalenceProspective StudiesReal-Time Polymerase Chain ReactionRespiratory Tract InfectionsSeasonsTertiary Care Centers

Identifiers

PMID27287764
PMCPMC7088036
OpenAlexW2419228861

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.