ArticleParasites & vectors2016
Virulence genes of Rickettsia rickettsii are differentially modulated by either temperature upshift or blood-feeding in tick midgut and salivary glands.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 36 citations in OpenAlex.
- Spotted fever group Rickettsia infection kinetics and cutaneous host response during single tick feeding events.PLoS pathogens · 2026Article
- Human infection with Candidatus Rickettsia jingxinensis: First identification and clinical characteristics.PLoS neglected tropical diseases · 2025Article
- Critical roles ofInfection and immunity · 2024Article
- Functional characterization ofFrontiers in cellular and infection microbiology · 2024Article
- Metabolism and physiology of pathogenic bacterial obligate intracellular parasites.Frontiers in cellular and infection microbiology · 2024Review
- Microbiome of Zoophytophagous Biological Control Agent Nesidiocoris tenuis.Microbial ecology · 2023Article
- Capybara density and climatic factors as modulators of Ehrlichia prevalence in questing ticks in the Iberá wetlands, Argentina.Scientific reports · 2023Article
- The survival of Amblyomma sculptum ticks upon blood-feeding depends on the expression of an inhibitor of apoptosis protein.Parasites & vectors · 2023Article
- Estimating the distribution ofEcosphere (Washington, D.C) · 2023Article
- Rickettsia Vaccine Candidate pVAX1-OmpB24 Stimulates TCD4+INF-γ+ and TCD8+INF-γ+ Lymphocytes in Autologous Co-Culture of Human Cells.Vaccines · 2023Article
- Unpacking the intricacies of Rickettsia-vector interactions.Trends in parasitology · 2021Review
- Differential expression of heat shock protein genes in Rhipicephalus sanguineus (Acari: Ixodidae) exposed to temperature and humidity variations.Parasitology research · 2021Article
- Cells within cells: Rickettsiales and the obligate intracellular bacterial lifestyle.Nature reviews. Microbiology · 2021Review
- Recent advances in understanding tick and rickettsiae interactions.Parasite immunology · 2021Review
- Isolate-Dependent Differences in Clinical, Pathological, and Transcriptional Profiles followingInfection and immunity · 2021Article
- Mechanisms Affecting the Acquisition, Persistence and Transmission ofMicroorganisms · 2020Review
- Comparative transcriptomic analysis of Rickettsia conorii during in vitro infection of human and tick host cells.BMC genomics · 2020Article
- Comparative Analysis of Infection byPathogens (Basel, Switzerland) · 2020Article
- Minimal Duration of Tick Attachment Sufficient for Transmission of Infectious Rickettsia rickettsii (Rickettsiales: Rickettsiaceae) by Its Primary Vector Dermacentor variabilis (Acari: Ixodidae): Duration of Rickettsial Reactivation in the Vector Revisited.Journal of medical entomology · 2020Article
- Francisella tularensis novicida infection competence differs in cell lines derived from United States populations of Dermacentor andersoni and Ixodes scapularis.Scientific reports · 2018Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRickettsia rickettsii, the etiological agent of Rocky Mountain spotted fever, is transmitted to humans by ticks. During tick feeding, R. rickettsii is exposed to both temperature elevation and components of the blood meal, which have previously been associated with the reactivation of its virulence. These environmental stimuli were also reported to modulate virulence genes of R. rickettsii infecting a set of organs of adult females of its natural vector, Amblyomma aureolatum.
methodsIn this study, we determined the effects of a temperature upshift, blood-feeding, and both stimuli simultaneously on the expression of 85 selected genes of R. rickettsii infecting either the midgut (MG) or salivary glands (SG) of male and female A. aureolatum by microfluidic high-throughput RT-qPCR. These two organs are key for acquisition of this bacterium by the tick and transmission to the vertebrate host, respectively.
resultsData showed that these environmental stimuli exert distinct effects on rickettsial transcription depending on the colonized organ and gender of the vector. Temperature upshift induced the majority of differentially expressed genes of R. rickettsii in tick SG, including tRNA synthetases encoding genes. On the contrary, blood-feeding downregulated most of differentially expressed genes in both organs, but induced type IV secretion system components and OmpB in tick MG. The combined effects of both stimuli resulted in a merged gene expression profile representing features of each stimulus analyzed independently, but was more similar to the profile induced by blood-feeding.
conclusionThe upregulation of the majority of differentially expressed genes in tick SG by temperature upshift suggests that this stimulus is important to prepare R. rickettsii for transmission to the vertebrate host. Blood-feeding, on the other hand, induced important virulence genes in the tick MG, which might be associated with colonization of the tick and transmission to the vertebrate host. The role of the proteins identified in this study must be addressed and might help to define future targets to block tick infection, thereby preventing RMSF. To our knowledge, this is the first transcriptional tissue-specific study of a virulent strain of R. rickettsii infecting a natural tick vector.
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