ArticleMicrobial cell factories2016
Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 58 citations in OpenAlex.
- Overexpression of msnA reprograms metabolism to enhance recombinant protein secretion across Aspergillus species.Microbial cell factories · 2026Article
- Efficient Extracellular Production of Insulin Glargine in Trichoderma reesei by an Integrative Secretion-Based Strategy.Microbial biotechnology · 2026Article
- The landscape of antibody production systems: recombinant expression for research, diagnostics and therapy.Frontiers in bioengineering and biotechnology · 2026Review
- Label-free analytical characterization of brazzein produced with the filamentous fungusFrontiers in bioengineering and biotechnology · 2025Article
- Enhanced heterologous gene expression in Trichoderma reesei by promoting multicopy integration.Applied microbiology and biotechnology · 2024Article
- Simultaneous Purification of Human Interferon Alpha-2b and Serum Albumin Using Bioprivileged Fluorinated Ionic Liquid-Based Aqueous Biphasic Systems.International journal of molecular sciences · 2024Article
- Heterologous protein production in filamentous fungi.Applied microbiology and biotechnology · 2023Review
- Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source.Microbial cell factories · 2023Article
- Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei.Microbial cell factories · 2023Article
- Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei.Bioresources and bioprocessing · 2022Article
- An overview on current molecular tools for heterologous gene expression in Trichoderma.Fungal biology and biotechnology · 2021Review
- Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production.Journal of industrial microbiology & biotechnology · 2021Article
- Molecular biology of coronaviruses: current knowledge.Heliyon · 2020Review
- Article
- Novel genetic tools that enable highly pure protein production in Trichoderma reesei.Scientific reports · 2019Article
- Enhancement of Cellulase Production inFrontiers in microbiology · 2019Article
- Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi.Applied microbiology and biotechnology · 2018Review
- A versatile system for fast screening and isolation ofBiotechnology for biofuels · 2018Article
- Article
- Scaling up and scaling down the production of galactaric acid from pectin using Trichoderma reesei.Microbial cell factories · 2017Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe filamentous fungus Trichoderma reesei has tremendous capability to secrete over 100 g/L of proteins and therefore it would make an excellent host system for production of high levels of therapeutic proteins at low cost. We have developed T. reesei strains suitable for production of therapeutic proteins by reducing the secreted protease activity. Protease activity has been the major hindrance to achieving high production levels. We have constructed a series of interferon alpha-2b (IFNα-2b) production strains with 9 protease deletions to gain knowledge for further strain development.
resultsWe have identified two protease deletions that dramatically improved the production levels. Deletion of the subtilisin protease slp7 and the metalloprotease amp2 has enabled production levels of IFNα-2b up to 2.1 and 2.4 g/L, respectively. With addition of soybean trypsin protease inhibitor the level of production improved to 4.5 g/L, with an additional 1.8 g/L still bound to the secretion carrier protein.
conclusionsHigh levels of IFNα-2b were produced using T. reesei strains with reduced protease secretion. Further strain development can be done to improve the production system by reducing protease activity and improving carrier protein cleavage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.