ArticleApplied and environmental microbiology2016
Mini-Tn7 Insertion in an Artificial attTn7 Site Enables Depletion of the Essential Master Regulator CtrA in the Phytopathogen Agrobacterium tumefaciens.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
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- DNA methylation by CcrM contributes to genome maintenance in the Agrobacterium tumefaciens plant pathogen.Nucleic acids research · 2024Article
- CRISPRi functional genomics in bacteria and its application to medical and industrial research.Microbiology and molecular biology reviews : MMBR · 2024Review
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- CRISPR-Associated Transposase for Targeted Mutagenesis in Diverse Proteobacteria.ACS synthetic biology · 2023Article
- Barcoding Populations of Pseudomonas fluorescens SBW25.Journal of molecular evolution · 2023Article
- Activation of ChvG-ChvI regulon by cell wall stress confers resistance to β-lactam antibiotics and initiates surface spreading in Agrobacterium tumefaciens.PLoS genetics · 2022Article
- A dicentric bacterial chromosome requires XerC/D site-specific recombinases for resolution.Current biology : CB · 2022Article
- Induction of AmpC-Mediated β-Lactam Resistance Requires a Single Lytic Transglycosylase in Agrobacterium tumefaciens.Applied and environmental microbiology · 2022Article
- Conformation and dynamic interactions of the multipartite genome inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Phenotypic and integrated analysis of a comprehensiveFrontiers in microbiology · 2022Article
- Unipolar Peptidoglycan Synthesis in themBio · 2021Article
- An improved, versatile and efficient modular plasmid assembly system for expression analyses of genes in Xanthomonas oryzae.Molecular plant pathology · 2021Article
- From macro to micro: a combined bioluminescence-fluorescence approach to monitor bacterial localization.Environmental microbiology · 2021Article
- Diversification of LytM Protein Functions in Polar Elongation and Cell Division ofFrontiers in microbiology · 2021Article
- Programmable Gene Knockdown in Diverse Bacteria Using Mobile-CRISPRi.Current protocols in microbiology · 2020Article
- The CtrA Regulon of Rhodobacter sphaeroides Favors Adaptation to a Particular Lifestyle.Journal of bacteriology · 2020Article
- Origin of a Core Bacterial Gene via Co-option and Detoxification of a Phage Lysin.Current biology : CB · 2019Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
unlabelledMechanistic studies of many processes in Agrobacterium tumefaciens have been hampered by a lack of genetic tools for characterization of essential genes. In this study, we used a Tn7-based method for inducible control of transcription from an engineered site on the chromosome. We demonstrate that this method enables tighter control of inducible promoters than plasmid-based systems and can be used for depletion studies. The method enables the construction of depletion strains to characterize the roles of essential genes in A. tumefaciens Here, we used the strategy to deplete the alphaproteobacterial master regulator CtrA and found that depletion of this essential gene results in dramatic rounding of cells, which become nonviable. IMPORTANCE: Agrobacterium tumefaciens is a bacterial plant pathogen and natural genetic engineer. Thus, studies of essential processes, including cell cycle progression, DNA replication and segregation, cell growth, and division, may provide insights for limiting disease or improving biotechnology applications.
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