Evidence map›Paper›PMID 27286678›Full record

ArticleActa biomaterialia2016

A bio-inspired hybrid nanosack for graft vascularization at the omentum.

Patrick T J Hwang, Dong-Jin Lim, Timothy Fee, Grant C Alexander, Ajay Tambralli, Adinarayana Andukuri, Liqun Tian, Wanxing Cui, Joel Berry, Shawn R Gilbert and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
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  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Patrick T J HwangDepartment of Biomedical Engineering, University of Alabama at Birmingham, AL 35294, USA.
Dong-Jin LimDepartment of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Timothy FeeDepartment of Biomedical Engineering, University of Alabama at Birmingham, AL 35294, USA.
Grant C AlexanderDepartment of Biomedical Engineering, University of Alabama at Birmingham, AL 35294, USA.
Ajay TambralliDepartment of Biomedical Engineering, University of Alabama at Birmingham, AL 35294, USA.
Adinarayana AndukuriDepartment of Biomedical Engineering, University of Alabama at Birmingham, AL 35294, USA.
Liqun TianDepartment of Surgery, University of Alabama at Birmingham, AL 35294, USA.
Wanxing CuiMedstar Georgetown Transplant Institute, Medstar Georgetown University Hospital, Washington DC 20007, USA.
Joel BerryDepartment of Biomedical Engineering, University of Alabama at Birmingham, AL 35294, USA.
Shawn R GilbertDepartment of Surgery, University of Alabama at Birmingham, AL 35294, USA.
Ho-Wook JunDepartment of Biomedical Engineering, University of Alabama at Birmingham, AL 35294, USA. Electronic address: hwjun@uab.edu.
University of Alabama at Birmingham · USBrigham and Women's Hospital · USGeorgetown University · USUniversity of Alabama at Birmingham Hospital · US

Funding

University of Alabama at Birmingham's Diabetes Research CenterP30DK079626 · NIDDK · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Stuart J Frank · 2013 to 2026
$19.5M
Cell therapy for diabetic peripheral neurovascular complicationsDP3DK094346 · NIDDK · EMORY UNIVERSITY · PI CHENG, XIAODONG, YOON, YOUNG-SUP · 2011 to 2011
$6.1M
Prohealing multifunctional endothelium nanomatrix coated stentR01HL125391 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI JUN, HO-WOOK · 2015 to 2019
$1.8M
A hybrid nanosack for the enhanced islet engraftment in the omentumR03EB017344 · NIBIB · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI JUN, HO-WOOK · 2013 to 2014
$143k
NHLBI NIH HHS R01 HL125391NIBIB NIH HHS R03 EB017344NIDDK NIH HHS DP3 DK094346NIDDK NIH HHS P30 DK079626
6 · The paper itself

Abstract

unlabelledFor three-dimensional tissue engineering scaffolds, the major challenges of hydrogels are poor mechanical integrity and difficulty in handling during implantation. In contrast, electrospun scaffolds provide tunable mechanical properties and high porosity; but, are limited in cell encapsulation. To overcome these limitations, we developed a "hybrid nanosack" by combination of a peptide amphiphile (PA) nanomatrix gel and an electrospun poly (ε-caprolactone) (ePCL) nanofiber sheet with porous crater-like structures. This hybrid nanosack design synergistically possessed the characteristics of both approaches. In this study, the hybrid nanosack was applied to enhance local angiogenesis in the omentum, which is required of tissue engineering scaffolds for graft survival. The ePCL sheet with porous crater-like structures improved cell and blood vessel penetration through the hybrid nanosack. The hybrid nanosack also provided multi-stage fibroblast growth factor-2 (FGF-2) release kinetics for stimulating local angiogenesis. The hybrid nanosack was implanted into rat omentum for 14days and vascularization was analyzed by micro-CT and immunohistochemistry; the data clearly demonstrated that both FGF-2 delivery and porous crater-like structures work synergistically to enhance blood vessel formation within the hybrid nanosack. Therefore, the hybrid nanosack will provide a new strategy for engineering scaffolds to achieve graft survival in the omentum by stimulating local vascularization, thus overcoming the limitations of current strategies. STATEMENT OF SIGNIFICANCE: For three-dimensional tissue engineering scaffolds, the major challenges of hydrogels are poor mechanical integrity and difficulty in handling during implantation. In contrast, electrospun scaffolds provide tunable mechanical properties and high porosity; but, are limited in cell encapsulation. To overcome these limitations, we developed a "hybrid nanosack" by combination of a peptide amphiphile (PA) nanomatrix gel and an electrospun poly (ε-caprolactone) (ePCL) nanofiber sheet with porous crater-like structures. This design synergistically possessed the characteristics of both approaches. In this study, the hybrid nanosack was applied to enhance local angiogenesis in the omentum, which is required of tissue engineering scaffolds for graft survival. The hybrid nanosack was implanted into rat omentum for 14days and vascularization was analyzed by micro-CT and immunohistochemistry. We demonstrate that both FGF-2 delivery and porous crater-like structures work synergistically to enhance blood vessel formation within the hybrid nanosack. Therefore, the hybrid nanosack will provide a new strategy for engineering scaffolds to achieve graft survival in the omentum by stimulating local vascularization, thus overcoming the limitations of current strategies.

Indexed as

AnimalsBiocompatible MaterialsEnzyme-Linked Immunosorbent AssayFibroblast Growth Factor 2HumansHuman Umbilical Vein Endothelial CellsImmunohistochemistryKineticsNanofibersNeovascularization, PhysiologicOmentumPlatelet Endothelial Cell Adhesion Molecule-1PolyestersPorosityRatsTissue ScaffoldsBiocompatible MaterialsFibroblast Growth Factor 2Platelet Endothelial Cell Adhesion Molecule-1polycaprolactonePolyestersCrater-like structureElectrospunHybrid nanosackOmentumTissue engineering

Identifiers

PMID27286678
PMCPMC4969099
OpenAlexW2418636916

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.