ArticleJournal of visualized experiments : JoVE2016
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
61 citing papers in PubMed.
- DNA-mimic for Specific Surface Functionalization of Zr-MOFs for Bacterial Targeting.Angewandte Chemie (International ed. in English) · 2026Article
- Super-Resolution Structured Illumination Microscopy to Study Endocytosis and Cellular Nanoparticle Uptake.ACS nano · 2026Review
- A High-Resolution Microscopy System for Biological Studies of Cold-Adapted Species Under Physiological Conditions.Small methods · 2025Article
- LED-based multicolor extended resolution transmission fluorescence microscopy.Journal of biomedical optics · 2025Article
- Concise Overview of Methodologies Employed in the Study of Bacterial DNA Replication.International journal of molecular sciences · 2025Review
- Optical sectioning methods in three-dimensional bioimaging.Light, science & applications · 2025Review
- Graphene Microelectrode Arrays, 4D Structured Illumination Microscopy, and a Machine Learning Spike Sorting Algorithm Permit the Analysis of Ultrastructural Neuronal Changes During Neuronal Signaling in a Model of Niemann-Pick Disease Type C.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane.Nature communications · 2024Article
- Fast volumetric multifocus structured illumination microscopy of subcellular dynamics in living cells.Biomedical optics express · 2024Article
- Fast, multicolour optical sectioning over extended fields of view with patterned illumination and machine learning.Biomedical optics express · 2024Article
- Super-Resolution Imaging of Neuronal Structures with Structured Illumination Microscopy.Bioengineering (Basel, Switzerland) · 2023Article
- Multiparametric Sensing of Outer Membrane Vesicle-Derived Supported Lipid Bilayers Demonstrates the Specificity of Bacteriophage Interactions.ACS biomaterials science & engineering · 2023Article
- Super-resolution imaging of neuronal structure with structured illumination microscopy.bioRxiv : the preprint server for biology · 2023Article
- Feasibility of Coacervate-Like Nanostructure for Instant Drug Nanoformulation.ACS applied materials & interfaces · 2023Article
- Machine learning assisted interferometric structured illumination microscopy for dynamic biological imaging.Nature communications · 2022Article
- Fluorescent Nanoparticles for Super-Resolution Imaging.Chemical reviews · 2022Review
- Nanoscale Features of Tunable Bacterial Outer Membrane Models Revealed by Correlative Microscopy.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Article
- Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of multiple cellular organelles during HSV-1 infection.PLoS pathogens · 2022Article
- Through the Eyes of Creators: Observing Artificial Molecular Motors.ACS nanoscience Au · 2022Review
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Optical super-resolution imaging with structured illumination microscopy (SIM) is a key technology for the visualization of processes at the molecular level in the chemical and biomedical sciences. Although commercial SIM systems are available, systems that are custom designed in the laboratory can outperform commercial systems, the latter typically designed for ease of use and general purpose applications, both in terms of imaging fidelity and speed. This article presents an in-depth guide to building a SIM system that uses total internal reflection (TIR) illumination and is capable of imaging at up to 10 Hz in three colors at a resolution reaching 100 nm. Due to the combination of SIM and TIRF, the system provides better image contrast than rival technologies. To achieve these specifications, several optical elements are used to enable automated control over the polarization state and spatial structure of the illumination light for all available excitation wavelengths. Full details on hardware implementation and control are given to achieve synchronization between excitation light pattern generation, wavelength, polarization state, and camera control with an emphasis on achieving maximum acquisition frame rate. A step-by-step protocol for system alignment and calibration is presented and the achievable resolution improvement is validated on ideal test samples. The capability for video-rate super-resolution imaging is demonstrated with living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.