ArticleNature communications2016
In vivo imaging of clock gene expression in multiple tissues of freely moving mice.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 50 citations in OpenAlex.
- Defining Clock Neurons Within Distributed Circadian Circuits Through Multiscale Technologies.Journal of biological rhythms · 2026Review
- Advances in monitoring mammalian circadian components and their rhythms using reporter systems.RSC chemical biology · 2026Review
- Circadian rhythms remain temperature compensated during a Q neuron-induced hibernation-like state in mice.PLoS biology · 2026Article
- HIV disrupts the lung molecular clock, leading to lung inflammation and features of emphysema.Communications biology · 2026Article
- Emerging Synthetic Bioluminescent Reactions for Non-Invasive Imaging of Freely Moving Animals.International journal of molecular sciences · 2024Review
- A Journey in the Brain's Clock: In Vivo Veritas?Biology · 2023Review
- Regulating macrophage-MSC interaction to optimize BMP-2-induced osteogenesis in the local microenvironment.Bioactive materials · 2023Article
- Analysis of the Anticipatory Behavior Formation Mechanism Induced by Methamphetamine Using a Single Hair.Cells · 2023Article
- Using bioluminescence to image gene expression and spontaneous behavior in freely moving mice.PloS one · 2023Article
- A real-time measurement system for gene expression rhythms from deep tissues of freely moving mice under light-dark conditions.Biochemistry and biophysics reports · 2022Article
- Cell-Type-Specific Circadian Bioluminescence Rhythms inJournal of biological rhythms · 2022Article
- Methods for Detecting PER2:LUCIFERASE Bioluminescence Rhythms in Freely Moving Mice.Journal of biological rhythms · 2022Article
- Ultrasmall compact CMOS imaging system for bioluminescence reporter-based live gene expression analysis.Journal of biomedical optics · 2021Article
- The liver-clock coordinates rhythmicity of peripheral tissues in response to feeding.Nature metabolism · 2021Article
- Circadian Chimeric Mice Reveal an Interplay Between the Suprachiasmatic Nucleus and Local Brain Clocks in the Control of Sleep and Memory.Frontiers in neuroscience · 2021Article
- Recurring circadian disruption alters circadian clock sensitivity to resetting.The European journal of neuroscience · 2020Article
- A standardized method to assess the endogenous activity and the light-response of the retinal clock in mammals.Molecular vision · 2020Article
- A single factor dominates the behavior of rhythmic genes in mouse organs.BMC genomics · 2019Article
- In vivo evaluation of the effect of lithium on peripheral circadian clocks by real-time monitoring of clock gene expression in near-freely moving mice.Scientific reports · 2019Article
- Evidence for Internal Desynchrony Caused by Circadian Clock Resetting.The Yale journal of biology and medicine · 2019Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clock genes are expressed throughout the body, although how they oscillate in unrestrained animals is not known. Here, we show an in vivo imaging technique that enables long-term simultaneous imaging of multiple tissues. We use dual-focal 3D tracking and signal-intensity calibration to follow gene expression in a target area. We measure circadian rhythms of clock genes in the olfactory bulb, right and left ears and cortices, and the skin. In addition, the kinetic relationship between gene expression and physiological responses to experimental cues is monitored. Under stable conditions gene expression is in phase in all tissues. In response to a long-duration light pulse, the olfactory bulb shifts faster than other tissues. In Cry1(-/-) Cry2(-/-) arrhythmic mice circadian oscillation is absent in all tissues. Thus, our system successfully tracks circadian rhythms in clock genes in multiple tissues in unrestrained mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.