ArticleJundishapur journal of microbiology2016
Molecular Identification of Mucor and Lichtheimia Species in Pure Cultures of Zygomycetes.
Article in Jundishapur journal of microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 24 citations in OpenAlex.
- Antifungal Properties ofPlants (Basel, Switzerland) · 2024Article
- Identification and Pathogenicity of a New Entomopathogenic Fungus, Mucor hiemalis (Mucorales: Mucorales), on the Root Maggot, Bradysia odoriphaga (Diptera: Sciaridae).Journal of insect science (Online) · 2022Article
- Difficulty of diagnosing a mucor-induced aneurysm arising in segment P4 of the posterior cerebral artery - A case report.Surgical neurology international · 2022Article
- Predisposing factors of important invasive fungal coinfections in COVID-19 patients: a review article.The Journal of international medical research · 2021Review
- Imaging features of perineural and perivascular spread in rapidly progressive rhino-orbital-cerebral mucormycosis: A case report and brief review of the literature.Surgical neurology international · 2021Article
- New Insights into Detection of a Dendrobine Compound From a Novel EndophyticFrontiers in microbiology · 2020Article
- Widespread Lichtheimia Infection in a Patient with Extensive Burns: Opportunities for Novel Antifungal Agents.Mycopathologia · 2019Article
- Identification of Mucorales isolates from soil using morphological and molecular methods.Current medical mycology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Mucorales are an important opportunistic fungi that can cause mucormycosis in immunocompromised patients. The fast and precise diagnosis of mucormycosis is very important because, if the diagnosis is not made early enough, dissemination often occurs. It is now well established that molecular methods such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) are feasible and reliable tools for the early and accurate diagnosis of mucormycosis agents.
objectivesThe present study was conducted to evaluate the validity of PCR-RFLP for the identification of Mucorales and some important Mucor and Lichtheimia species in pure cultures of Zygomycetes. MATERIALS AND
methodsSpecific sense and anti-sense primers were used to amplify the Mucorales, Mucor, and Lichtheimia DNA. The PCR products were digested by AfIII, XmnI, and AcII restriction enzymes, and the resultant restriction pattern was analyzed.
resultsOn the basis of the molecular and morphological data, we identified Mucor plumbeus (10.83%), M. circinelloides (9.17%), Lichtheimia corymbifera (9.17%), M. racemosus (5.83%), M. ramosissimus (3.33%), and L. blakesleeana (0.83%).
conclusionsIt seems that PCR-RFLP is a suitable technique for the identification of Mucorales at the species level.
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