Evidence map›Paper›PMID 27284306›Full record

ArticleExperimental and therapeutic medicine2016

Hypoxia induces autophagy in cardiomyocytes via a hypoxia-inducible factor 1-dependent mechanism.

Lan Gui, Batu Liu, Guang Lv

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental and therapeutic medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 1 pooled it
4.5field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.

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  8. Regulation of autophagy of the heart in ischemia and reperfusion.Apoptosis : an international journal on programmed cell death · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Lan GuiDepartment of Basic Theory, Sports Institute of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028041, P.R. China; Institute of Mongolian Genome and Genetic Diseases, Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
Batu LiuSports Department, Sports Institute of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
Guang LvInstitute of Pathogenic Microbiology and Immunology, Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
Inner Mongolia University for Nationalities · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hypoxia frequently accompanies such vascular disorders as atherosclerosis, thrombosis and ischemia/reperfusion injury. Myocardial ischemia/reperfusion, in particular, is a major contributor to cardiomyocyte impairment. Autophagy is a dynamic, self-catabolic process that has been implicated in a wide range of physiological processes and the pathogenesis of diverse diseases. The aim of the present study was to investigate the promotion of autophagy by hypoxia in a rat H9c2 heart cell line and determine the regulatory role of hypoxia-inducible factor 1 (HIF-1) in the hypoxia-induced autophagy in H9c2 cells, using quantitative green fluorescent protein-microtubule-associated protein 1 light chain 3 analysis and electron microscopy of autophagic vesicles. In addition, western blot and quantitative polymerase chain reaction analysis of autophagy-associated markers was conducted. In addition, the role of HIF-1-mediated autophagy in the hypoxia-induced impairment of H9c2 cells was examined, as a measure of cellular viability, using an MTT assay. The results demonstrated that autophagy was induced in H9c2 cells under hypoxia, and the autophagy induction triggered by hypoxia could be enhanced by HIF-1α overexpression and inhibited by HIF-1α knockdown. Furthermore, the HIF-1-mediated autophagy ameliorated the reduction in the H9c2 cell viability induced by hypoxia. These findings provide a novel insight into the hypoxic-ischemic injury to cardiomyocytes and give evidence for the occurrence of HIF-1-mediated autophagy in myocardial ischemia.

Indexed as

autophagycardiomyocyteshypoxiahypoxia-inducible factor 1α

Identifiers

PMID27284306
PMCPMC4887955
OpenAlexW2310478501

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.