ArticleScience (New York, N.Y.)2016
Molecular recordings by directed CRISPR spacer acquisition.
Article in Science (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
113 citing papers in PubMed, 246 citations in OpenAlex.
- Detectrons convert transient RNA sequences into stable DNA barcodes for high-throughput analysis of RNA-dependent processes.Nature biotechnology · 2026Article
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- Genomic repeats for single-cell molecular recording.bioRxiv : the preprint server for biology · 2026Article
- De novo design of potent CRISPR-Cas13 inhibitors.Nature chemical biology · 2026Article
- Article
- Insights into spacer acquisition of the type V-A CRISPR-Cas system of Francisella novicida U112.Nucleic acids research · 2026Article
- Genetic recording and in situ readout of single-cell signaling memory.Nature chemical biology · 2026Article
- Information storage across a microbial community using universal RNA barcoding.Nature biotechnology · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- CRISPR-based genetic tools for the study of host-microbe interactions.Infection and immunity · 2025Review
- MitoScribe single-cell molecular recorder logs graded signaling dynamics into mitochondrial DNA.bioRxiv : the preprint server for biology · 2025Article
- BioFuse: A programmable timer switch of gene expression.Science advances · 2025Article
- Deep mutational scanning identifies Cas1 and Cas2 variants that enhance type II-A CRISPR-Cas spacer acquisition.Nature communications · 2025Article
- Open-ended molecular recording of sequential cellular events into DNA.Nature chemical biology · 2025Article
- SspA is a transcriptional regulator of CRISPR adaptation in E. coli.Nucleic acids research · 2025Article
- Multifunctional Au-Ag-Cr Nanocomposites: From Multiplex Sensing and Advanced Logic Computing to Scalable Information Protection.Research (Washington, D.C.) · 2025Article
- From Random Perturbation to Precise Targeting: A Comprehensive Review of Methods for Studying Gene Function inJournal of fungi (Basel, Switzerland) · 2024Review
- Post-translational digital data encoding into the genomes of mammalian cell populations.bioRxiv : the preprint server for biology · 2024Article
- Reconstructing signaling history of single cells with imaging-based molecular recording.bioRxiv : the preprint server for biology · 2024Article
- Molecular recording using DNA Typewriter.Nature protocols · 2024Review
53 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ability to write a stable record of identified molecular events into a specific genomic locus would enable the examination of long cellular histories and have many applications, ranging from developmental biology to synthetic devices. We show that the type I-E CRISPR (clustered regularly interspaced short palindromic repeats)-Cas system of Escherichia coli can mediate acquisition of defined pieces of synthetic DNA. We harnessed this feature to generate records of specific DNA sequences into a population of bacterial genomes. We then applied directed evolution so as to alter the recognition of a protospacer adjacent motif by the Cas1-Cas2 complex, which enabled recording in two modes simultaneously. We used this system to reveal aspects of spacer acquisition, fundamental to the CRISPR-Cas adaptation process. These results lay the foundations of a multimodal intracellular recording device.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.