ArticleRNA (New York, N.Y.)2016
The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNATyr.
Article in RNA (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- The mitochondrial proteome of diplonemids: from conventional pathways to eccentric RNA editing and transcript processing.BMC genomics · 2025Article
- Systematic Analysis of Diverse Polynucleotide Kinase Clp1 Family Proteins in Eukaryotes: Three Unique Clp1 Proteins of Trypanosoma brucei.Journal of molecular evolution · 2023Article
- New insights into RNA processing by the eukaryotic tRNA splicing endonuclease.The Journal of biological chemistry · 2023Review
- tRNARNA (New York, N.Y.) · 2023Article
- The life and times of a tRNA.RNA (New York, N.Y.) · 2023Review
- Three tRNA nuclear exporters in S. cerevisiae: parallel pathways, preferences, and precision.Nucleic acids research · 2022Article
- Making Sense of "Nonsense" and More: Challenges and Opportunities in the Genetic Code Expansion, in the World of tRNA Modifications.International journal of molecular sciences · 2022Review
- Trafficking and/or division: Distinct roles of nucleoporins based on their location within the nuclear pore complex.RNA biology · 2022Article
- Dynamic queuosine changes in tRNA couple nutrient levels to codon choice in Trypanosoma brucei.Nucleic acids research · 2021Article
- Identification and Functional Characterization of Divergent 3'-Phosphate tRNA Ligase FromFrontiers in cellular and infection microbiology · 2021Article
- The general mRNA exporters Mex67 and Mtr2 play distinct roles in nuclear export of tRNAs in Trypanosoma brucei.Nucleic acids research · 2019Article
- Structure and two-metal mechanism of fungal tRNA ligase.Nucleic acids research · 2019Article
- How the intracellular partitioning of tRNA and tRNA modification enzymes affects mitochondrial function.IUBMB life · 2018Review
- tRNA dynamics between the nucleus, cytoplasm and mitochondrial surface: Location, location, location.Biochimica et biophysica acta. Gene regulatory mechanisms · 2018Review
- Genetic bypass of essential RNA repair enzymes in budding yeast.RNA (New York, N.Y.) · 2018Article
- Retrograde nuclear transport from the cytoplasm is required for tRNARNA biology · 2018Article
- The role of intracellular compartmentalization on tRNA processing and modification.RNA biology · 2018Review
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
Trypanosoma brucei, the etiologic agent of sleeping sickness, encodes a single intron-containing tRNA, tRNA(Tyr), and splicing is essential for its viability. In Archaea and Eukarya, tRNA splicing requires a series of enzymatic steps that begin with intron cleavage by a tRNA-splicing endonuclease and culminates with joining the resulting tRNA exons by a splicing tRNA ligase. Here we explored the function of TbTrl1, the T. brucei homolog of the yeast Trl1 tRNA ligase. We used a combination of RNA interference and molecular biology approaches to show that down-regulation of TbTrl1 expression leads to accumulation of intron-containing tRNA(Tyr) and a concomitant growth arrest at the G1 phase. These defects were efficiently rescued by expression of an "intronless" version of tRNA(Tyr) in the same RNAi cell line. Taken together, these experiments highlight the crucial importance of the TbTrl1 for tRNA(Tyr) maturation and viability, while revealing tRNA splicing as its only essential function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.