Evidence map›Paper›PMID 27284111›Full record

ArticleDiabetes2016

Hsp20-Mediated Activation of Exosome Biogenesis in Cardiomyocytes Improves Cardiac Function and Angiogenesis in Diabetic Mice.

Xiaohong Wang, Haitao Gu, Wei Huang, Jiangtong Peng, Yutian Li, Liwang Yang, Dongze Qin, Kobina Essandoh, Yigang Wang, Tianqing Peng and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diabetes, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 154 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
154citing papers in PubMed, 4 pooled it
13.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

154 citing papers in PubMed, 4 syntheses or guidelines pooled it, 239 citations in OpenAlex.

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  11. Diabetes, Protein Misfolding, and Heat Stress: Molecular Insights and Translational Perspectives.TH open : companion journal to thrombosis and haemostasis · 2026
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94 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 3 countries.

Xiaohong WangDepartment of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH.
Haitao GuDepartment of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH.
Wei HuangDepartment of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH.
Jiangtong PengDepartment of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH Department of Cardiovascular Diseases, Tongji Medical College Union Hospital, Huazhong University of Science and Technology, Wuhan, China.
Yutian LiDepartment of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH.
Liwang YangDepartment of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH.
Dongze QinDepartment of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH.
Kobina EssandohDepartment of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH.
Yigang WangDepartment of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH.
Tianqing PengCritical Illness Research, Lawson Health Research Institute, Ontario, Canada.
Guo-Chang FanDepartment of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH fangg@ucmail.uc.edu.ORCID 0000-0002-0439-8277
University of Cincinnati Medical Center · USLawson Health Research Institute · CAUnion Hospital · CN

Funding

Roles of Hsp20-Exosomes in Myocardial AngiogenesisR01HL087861 · NHLBI · UNIVERSITY OF CINCINNATI · PI FAN, GUO-CHANG · 2007 to 2017
$4.2M
Duplex miR-223 and Exosomes in SepsisR01GM112930 · NIGMS · UNIVERSITY OF CINCINNATI · PI FAN, GUO-CHANG · 2015 to 2018
$1.2M
NHLBI NIH HHS R01 HL087861NIGMS NIH HHS R01 GM112930
6 · The paper itself

Abstract

Decreased heat shock protein (Hsp) expression in type 1 and type 2 diabetes has been implicated as a primary factor contributing to diabetes-induced organ damage. We recently showed that diabetic cardiomyocytes could release detrimental exosomes, which contain lower levels of Hsp20 than normal ones. To investigate whether such detrimental exosomes could be modified in cardiomyocytes by raising Hsp20 levels to become protective, we used a transgenic (TG) mouse model with cardiac-specific overexpression of Hsp20. TG and control wild-type (WT) mice were injected with streptozotocin (STZ) to induce diabetes. We observed that overexpression of Hsp20 significantly attenuated STZ-caused cardiac dysfunction, hypertrophy, apoptosis, fibrosis, and microvascular rarefaction. Moreover, Hsp20-TG cardiomyocytes exhibited an increased generation/secretion of exosomes by direct interaction of Hsp20 with Tsg101. Of importance, exosomes derived from TG cardiomyocytes encased higher levels of Hsp20, p-Akt, survivin, and SOD1 than WT exosomes and protected against in vitro hyperglycemia-triggered cell death, as well as in vivo STZ-induced cardiac adverse remodeling. Last, blockade of exosome generation by GW4869 remarkably offset Hsp20-mediated cardioprotection in diabetic mice. Our results indicate that elevation of Hsp20 in cardiomyocytes can offer protection in diabetic hearts through the release of instrumental exosomes. Thus, Hsp20-engineered exosomes might be a novel therapeutic agent for diabetic cardiomyopathy.

Indexed as

Aniline CompoundsAnimalsBenzylidene CompoundsCell MovementCell ProliferationCells, CulturedCollagen Type ICollagen Type IIIDiabetes Mellitus, ExperimentalEndothelial CellsExosomesHeartHSP20 Heat-Shock ProteinsMaleMiceMice, TransgenicAniline CompoundsBenzylidene CompoundsCollagen Type ICollagen Type IIIGW 4869HSP20 Heat-Shock ProteinsPlatelet Endothelial Cell Adhesion Molecule-1Reactive Oxygen SpeciesSod1 protein, mouseSuperoxide Dismutase-1

Identifiers

PMID27284111
PMCPMC5033265
OpenAlexW2416609191

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.