Evidence map›Paper›PMID 27284092›Full record

ArticleThe Journal of infectious diseases2016

Toll-like Receptor Expression and Signaling in Peripheral Blood Mononuclear Cells Correlate With Clinical Outcomes in Acute Hepatitis C Virus Infection.

Swee Lin G Chen Yi Mei, Jodie Burchell, Narelle Skinner, Rosie Millen, Gail Matthews, Margaret Hellard, Gregory J Dore, Paul V Desmond, Vijaya Sundararajan, Alexander J Thompson and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infectious diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 7 institutions in 3 countries.

Swee Lin G Chen Yi MeiDepartment of Gastroenterology, St Vincent's Hospital Immunology Research Centre, Department of Medicine, St Vincent's Hospital and the University of Melbourne.
Jodie BurchellCentre of Research Excellence, Department of Medicine, St Vincent's Hospital, Melbourne.
Narelle SkinnerImmunology Research Centre, Department of Medicine, St Vincent's Hospital and the University of Melbourne.
Rosie MillenImmunology Research Centre, Department of Medicine, St Vincent's Hospital and the University of Melbourne.
Gail MatthewsKirby Institute, University of New South Wales, Sydney.
Margaret HellardCentre for Population Health, Burnet Institute Department of Infectious Diseases, the Alfred Hospital, Melbourne, Australia.
Gregory J DoreKirby Institute, University of New South Wales, Sydney.
Paul V DesmondDepartment of Gastroenterology, St Vincent's Hospital.
Vijaya SundararajanCentre of Research Excellence, Department of Medicine, St Vincent's Hospital, Melbourne.
Alexander J ThompsonDepartment of Gastroenterology, St Vincent's Hospital Immunology Research Centre, Department of Medicine, St Vincent's Hospital and the University of Melbourne.
Kumar VisvanathanDepartment of Gastroenterology, St Vincent's Hospital Immunology Research Centre, Department of Medicine, St Vincent's Hospital and the University of Melbourne.
Joe SasadeuszDepartment of Infectious Diseases, Royal Melbourne Hospital.
Saint Vincent's Catholic Medical Center · USSt Vincent's Hospital · AUUNSW Sydney · AUSt. Vincent's Hospital · KRThe Alfred Hospital · AUThe Royal Melbourne Hospital · AUUniversity of Melbourne · AU

Funding

Treatment of recently acquired hepatitis C virus infectionR01DA015999 · NIDA · UNIVERSITY OF NEW SOUTH WALES · PI DORE, GREGORY JOHN · 2003 to 2014
$3.7M
NIDA NIH HHS R01 DA015999
6 · The paper itself

Abstract

backgroundMechanisms by which spontaneous clearance of acute hepatitis C occurs are unclear. A critical role for the innate immune system and IFNL4 polymorphisms has been proposed. This study investigates whether Toll-like receptor (TLR) expression and signaling during acute hepatitis C correlates with clinical outcomes.

methodsParticipants identified from the Australian Trial in Acute Hepatitis C and the Networks study were followed longitudinally from the time of diagnosis of acute hepatitis C. Peripheral blood mononuclear cells (PBMCs) and plasma were collected at and 2 time points after diagnosis. At each time point, TLR2, TLR4, and CD86 expression on peripheral blood monocytes, natural killer (NK) cells, and NK T cells was measured, as well as the response of PBMCs to stimulation with TLR ligands. Cytokine and chemokine levels were measured in stimulated PBMCs and plasma.

resultsWe identified 20 participants with acute hepatitis C (10 with hepatitis C virus [HCV] monoinfection and 10 with HCV and human immunodeficiency virus coinfection). Eleven participants (55%) spontaneously cleared HCV. Acute hepatitis C and spontaneous clearance was associated with lower TLR4 expression on monocytes (P = .009) and NK cells (P = .029). Acute hepatitis C and spontaneous clearance was also associated with a reduced interferon γ response to TLR4 (P = .038) and TLR7/8 stimulation (P = .035), a reduced interleukin 6 response to TLR7/8 stimulation (P = .037), and reduced IFN-γ-inducible protein 10 (IP-10) response to TLR2 stimulation (P = .042). Lower plasma IP-10 levels were associated with spontaneous clearance (P = .001).

conclusionsThese findings implicate TLR4 signaling as playing a critical role in the outcome of acute hepatitis C.

Indexed as

Signal TransductionAdultAustraliaB7-2 AntigenCytokinesFemaleHepatitis CHumansLeukocytes, MononuclearLongitudinal StudiesMaleMiddle AgedToll-Like Receptor 2Toll-Like Receptor 4Treatment OutcomeB7-2 AntigenCD86 protein, humanCytokinesTLR2 protein, humanTLR4 protein, humanToll-Like Receptor 2Toll-Like Receptor 4cytokineshepatitis Ctoll-like receptors

Identifiers

PMID27284092
PMCPMC4978374
OpenAlexW2415136511

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.