ArticleScientific reports2016
Resolving new ultrastructural features of cytokinetic abscission with soft-X-ray cryo-tomography.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Loss of CHMP2A implicates an ordered assembly of ESCRT-III proteins during cytokinetic abscission.Molecular biology of the cell · 2025Article
- Viral vaccines promote endoplasmic reticulum stress-induced unfolding protein response in teleost erythrocytes.European journal of cell biology · 2025Article
- Review
- Laboratory Liquid-Jet X-ray Microscopy and X-ray Fluorescence Imaging for Biomedical Applications.International journal of molecular sciences · 2024Article
- 3D reconstruction of the cerebellar germinal layer reveals tunneling connections between developing granule cells.Science advances · 2023Article
- Mechanics and regulation of cytokinetic abscission.Frontiers in cell and developmental biology · 2022Review
- AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.PLoS genetics · 2021Article
- Article
- Review
- A Structural View on ESCRT-Mediated Abscission.Frontiers in cell and developmental biology · 2020Review
- The postmitotic midbody: Regulating polarity, stemness, and proliferation.The Journal of cell biology · 2019Review
- X-ray tomography shows the varying three-dimensional morphology of gold nanoaggregates in the cellular ultrastructure.Nanoscale advances · 2019Article
- Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Precise and economic FIB/SEM for CLEM: with 2 nm voxels through mitosis.Histochemistry and cell biology · 2018Article
- Dividing the Archaeal Way: The Ancient Cdv Cell-Division Machinery.Frontiers in microbiology · 2018Review
- Membrane remodeling during embryonic abscission inThe Journal of cell biology · 2017Article
- Mechanisms and functions of extracellular vesicle release in vivo-What we can learn from flies and worms.Cell adhesion & migration · 2017Review
- Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell.Biology of the cell · 2017Review
Corrections and comments
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Authors and funding
7 authors.
Funding
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Abstract
Mammalian cytokinetic abscission is mediated by the ESCRT membrane fission machinery. While much has been clarified on the topology and kinetics of abscission through high-resolution microscopy, key questions regarding the mechanism of abscission remain open. Here we apply cryogenic soft-X-ray tomography to elucidate new ultrastructural details in the intercellular membrane bridge connecting cells undergoing abscission. In particular, we resolve defined ring-like structures inside the midbody dark zone that have been inaccessible to EM, and identify membrane extrusions at the abscission sites. In cells at late stages of abscission we resolve a complex array of helical spirals, extending the structural information obtained by EM. Our results highlight the advantages of soft-X-ray tomography and emphasize the importance of using complementary approaches for characterizing cellular structures. Notably, by providing new structural data from intact cells we present a realistic view on the topology of abscission and suggest new mechanistic models for ESCRT mediated abscission.
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