ArticleTicks and tick-borne diseases2016
Comparative value of blood and skin samples for diagnosis of spotted fever group rickettsial infection in model animals.
Article in Ticks and tick-borne diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Turning urban wildlife mortality into a surveillance tool: Detection of vector-borne pathogens in carcasses of hedgehogs, squirrels, and blackbirds.One health (Amsterdam, Netherlands) · 2026Article
- Simultaneous serological assessment of four zoonotic rickettsiae among dogs near the United States-Mexico border.PLoS neglected tropical diseases · 2026Article
- A rapid isothermal RPA-CRISPR/Cas12a assay for detection ofFrontiers in microbiology · 2026Article
- Experimental infection of wild boars (Sus scrofa) with Rickettsia rickettsii and evaluation of the transmission potential to Amblyomma sculptum ticks.Parasites & vectors · 2025Article
- Duplex Reverse-Transcription Real-Time Polymerase Chain Reaction Assay Targeting 23S rRNA Single Nucleotide Polymorphisms for the Detection of Flea-Borne Rickettsioses.The American journal of tropical medicine and hygiene · 2024Article
- Spatially limited pathogen pollution in an invasive tick and host system.Biological invasions · 2024Article
- Evaluating the Clinical and Immune Responses to Spotted Fever Rickettsioses in the Guinea Pig-Tick-Rickettsia System.Current protocols · 2022Article
- Brown Dog Tick (International journal of environmental research and public health · 2022Article
- An Update on the Laboratory Diagnosis ofPathogens (Basel, Switzerland) · 2021Review
- Role of reptiles and associated arthropods in the epidemiology of rickettsioses: A one health paradigm.PLoS neglected tropical diseases · 2021Article
- The guinea pig model for tick-borne spotted fever rickettsioses: A second look.Ticks and tick-borne diseases · 2020Review
- Cutaneous Immunoprofiles of Three Spotted Fever Group Rickettsia Cases.Infection and immunity · 2020Article
- Minimal Duration of Tick Attachment Sufficient for Transmission of Infectious Rickettsia rickettsii (Rickettsiales: Rickettsiaceae) by Its Primary Vector Dermacentor variabilis (Acari: Ixodidae): Duration of Rickettsial Reactivation in the Vector Revisited.Journal of medical entomology · 2020Article
- Vector Tick Transmission Model of Spotted Fever Rickettsiosis.The American journal of pathology · 2019Article
- Hemogregarine and Rickettsial infection in ticks of toads from northeastern Colombia.International journal for parasitology. Parasites and wildlife · 2018Article
- Neglected aspects of tick-borne rickettsioses.Parasites & vectors · 2018Review
- Article
- Vector competence of Amblyomma americanum (Acari: Ixodidae) for Rickettsia rickettsii.Ticks and tick-borne diseases · 2017Article
- Unique Strain of Rickettsia parkeri Associated with the Hard Tick Dermacentor parumapertus Neumann in the Western United States.Applied and environmental microbiology · 2017Article
- Assessment of Domestic Goats as Models for Experimental and Natural Infection with the North American Isolate of Rickettsia slovaca.PloS one · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The definitive diagnosis of spotted fever group (SFG) rickettsioses in humans is challenging due to the retrospective nature and cross reactivity of the serological methods and the absence of reliable and consistent samples for molecular diagnostics. Existing data indicate the transient character of bacteremia in experimentally infected animals. The ability of arthropod vectors to acquire rickettsial infection from the laboratory animals in the absence of systemic infection and known tropism of rickettsial agents to endothelial cells of peripheral blood vessels underline the importance of local infection and consequently the diagnostic potential of skin samples. In order to evaluate the diagnostic sensitivity of rickettsial DNA detection in blood and skin samples, we compared results of PCR testing in parallel samples collected from model laboratory animals infected with Rickettsia rickettsii, Rickettsia parkeri and Rickettsia slovaca-like agent at different time points after infection. Skin samples were collected from ears - away from the site of tick placement and without eschars. Overall, testing of skin samples resulted in a higher proportion of positive results than testing of blood samples. Presented data from model animals demonstrates that testing of skin samples from sites of rickettsial proliferation can provide definitive molecular diagnosis of up to 60-70% of tick-borne SFG rickettsial infections during the acute stage of illness. Detection of pathogen DNA in cutaneous samples is a valuable alternative to blood-PCR at least in model animals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.