ArticleOncotarget2016
Immunoproteomic identification of MbovP579, a promising diagnostic biomarker for serological detection of Mycoplasma bovis infection.
Article in Oncotarget, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Use of recombinant proteins for the diagnosis and prevention ofFrontiers in veterinary science · 2024Pooled it
- Serological Detection of Antibodies AgainstVeterinary sciences · 2026Article
- Identification ofMicroorganisms · 2026Article
- Article
- MbovP0725, a secreted serine/threonine phosphatase, inhibits the host inflammatory response and affects metabolism inmSystems · 2024Article
- Point-of-care potentials of lateral flow-based field screening forBiology methods & protocols · 2024Review
- Novel mycoplasma nucleomodulin MbovP475 decreased cell viability by regulating expression of CRYAB and MCF2L2.Virulence · 2022Article
- A novel phage-displayed MilA ELISA for detection of antibodies against Myc. bovis in bovine milk.Journal of applied microbiology · 2022Article
- Mycoplasmas as Host Pantropic and Specific Pathogens: Clinical Implications, Gene Transfer, Virulence Factors, and Future Perspectives.Frontiers in cellular and infection microbiology · 2022Review
- Proteomics approaches: A review regarding an importance of proteome analyses in understanding the pathogens and diseases.Frontiers in veterinary science · 2022Review
- Clinicopathological and Sero-Molecular Detection ofVeterinary medicine international · 2022Article
- Secreted MbovP0145 Promotes IL-8 Expression through Its Interactive β-Actin and MAPK Activation and Contributes to Neutrophil Migration.Pathogens (Basel, Switzerland) · 2021Article
- Novel Secreted Protein ofFrontiers in immunology · 2021Article
- Comparative Secretome Analyses ofFrontiers in veterinary science · 2021Article
- Immunoinformatics and analysis of antigen distribution of Ureaplasma diversum strains isolated from different Brazilian states.BMC veterinary research · 2020Article
- In Silico Identification of Novel Immunogenic Secreted Proteins ofPathogens (Basel, Switzerland) · 2020Article
- Antibodies Specific to Membrane Proteins Are Effective in Complement-Mediated Killing of Mycoplasma bovis.Infection and immunity · 2019Article
- Novel Candidates for Vaccine Development AgainstVaccines · 2019Review
- Proteomics identification and characterization of MbovP730 as a potential DIVA antigen ofOncotarget · 2018Article
- Identification of potential urine proteins and microRNA biomarkers for the diagnosis of pulmonary tuberculosis patients.Emerging microbes & infections · 2018Article
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A lack of knowledge regarding the antigenic properties of Mycoplasma bovis proteins prevents the effective control of bovine infections using immunological approaches. In this study, we detected and characterized a specific and sensitive M. bovis diagnostic biomarker. After M. bovis total proteins and membrane fractions were separated with two dimensional gel electrophoresis, proteins reacting with antiserawere detected using MALDI-TOF MS. Thirty-nine proteins were identified, 32 of which were previously unreported. Among them, immunoinformatics predicted eight antigens, encoded by Mbov_0106, 0116, 0126, 0212, 0275, 0579, 0739, and 0789, to have high immunological value. These genes were expressed in E. coli after mutagenesis of UGA to UGG using overlap extension PCR. A lipoprotein, MbovP579, encoded by a functionally unknown gene, was a sensitive and specific antigen for detection of antibodies in sera from both M. bovis-infected and vaccinated cattle. The specificity of MbovP579 was confirmed by its lack of cross-reactivity with other mycoplasmas, including Mycoplasma agalactiae. An iELISA based on rMbovP579 detected seroconversion 7 days post-infection (dpi). The ELISA had sensitivity of 90.2% (95% CI: 83.7%, 94.3%) and a specificity of 97.8% (95% CI: 88.7%, 99.6%) with clinical samples. Additional comparative studies showed that both diagnostic and analytic sensitivities of the ELISA were higher than those of a commercially available kit (p<0.01). We have thus detected and characterized the novel antigen, MbovP579, and established an rMbovP579-based ELISA as a highly sensitive and specific method for the early diagnosis of M. bovis infection.
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