Evidence map›Paper›PMID 27281571›Full record

ArticleCell host & microbe2016

Salmonella Mitigates Oxidative Stress and Thrives in the Inflamed Gut by Evading Calprotectin-Mediated Manganese Sequestration.

Vladimir E Diaz-Ochoa, Diana Lam, Carlin S Lee, Suzi Klaus, Judith Behnsen, Janet Z Liu, Nicholas Chim, Sean-Paul Nuccio, Subodh G Rathi, Jennifer R Mastroianni and 9 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell host & microbe, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.

0numbers the graph read from it
0cells of the map it votes in
110citing papers in PubMed
10.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

110 citing papers in PubMed, 164 citations in OpenAlex.

  1. Review
  2. Article
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  6. Aspartate aminotransferase is required forInfection and immunity · 2026
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  7. Aspartate aminotransferase is required forbioRxiv : the preprint server for biology · 2026
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  8. Review
  9. Infection strategies ofGut microbes reports · 2026
    Review
  10. Article
  11. SLC11A2 affects nutritional immunity in the gut epithelium.bioRxiv : the preprint server for biology · 2025
    Article
  12. Article
  13. Review
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  15. Article
  16. Article
  17. Article
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  19. Review
  20. mBio · 2025
    Article

50 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 5 institutions in 2 countries.

Vladimir E Diaz-OchoaDepartment of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697-4025, USA; Institute for Immunology, University of California, Irvine, Irvine, CA 92697-4120, USA.
Diana LamDepartment of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697-4025, USA; Institute for Immunology, University of California, Irvine, Irvine, CA 92697-4120, USA.
Carlin S LeeDepartment of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697-4025, USA; Institute for Immunology, University of California, Irvine, Irvine, CA 92697-4120, USA.
Suzi KlausDepartment of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697-4025, USA; Institute for Immunology, University of California, Irvine, Irvine, CA 92697-4120, USA.
Judith BehnsenDepartment of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697-4025, USA; Institute for Immunology, University of California, Irvine, Irvine, CA 92697-4120, USA.
Janet Z LiuDepartment of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697-4025, USA; Institute for Immunology, University of California, Irvine, Irvine, CA 92697-4120, USA.
Nicholas ChimDepartment of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697-3900, USA.
Sean-Paul NuccioDepartment of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697-4025, USA; Institute for Immunology, University of California, Irvine, Irvine, CA 92697-4120, USA.
Subodh G RathiDepartment of Biochemistry and Chemistry, Vanderbilt University, Nashville, TN 37232-8725, USA.
Jennifer R MastroianniDepartment of Pathology and Laboratory Medicine, University of Southern California, Los Angeles, CA 90089-9092, USA.
Robert A EdwardsDepartment of Pathology and Laboratory Medicine, University of California, Irvine, Irvine, CA 92697-4800, USA.
Christina M JacoboDepartment of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697-4025, USA; Institute for Immunology, University of California, Irvine, Irvine, CA 92697-4120, USA.
Mauro CerasiDepartment of Biology, University of Rome, Tor Vergata, 00173 Roma, Italy.
Andrea BattistoniDepartment of Biology, University of Rome, Tor Vergata, 00173 Roma, Italy.
André J OuelletteDepartment of Pathology and Laboratory Medicine, University of Southern California, Los Angeles, CA 90089-9092, USA.
Celia W GouldingDepartment of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697-3900, USA; Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA 92697-3958, USA.
Walter J ChazinDepartment of Biochemistry and Chemistry, Vanderbilt University, Nashville, TN 37232-8725, USA.
Eric P SkaarDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232-2363, USA; Tennessee Valley Healthcare System, US Department of Veterans Affairs, Nashville, TN 37212, USA.
Manuela RaffatelluDepartment of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697-4025, USA; Institute for Immunology, University of California, Irvine, Irvine, CA 92697-4120, USA. Electronic address: manuelar@uci.edu.
University of California, Irvine · USUniversity of Rome Tor Vergata · ITUniversity of Southern California · USVanderbilt University · USUnited States Department of Veterans Affairs · US

Funding

Vulnerabilities in Mycobacterial Cell-Wall BiogenesisP01AI095208 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI SACCHETTINI, JAMES C · 2012 to 2022
$19.8M
MINORITY BIOMEDICAL RESEARCHERS PROGRAMR25GM055246 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI MOTA-BRAVO, LUIS M · 1996 to 2022
$15.3M
Host-mediated zinc sequestration during Acinetobacter baumannii infectionR01AI101171 · NIAID · VANDERBILT UNIVERSITY MEDICAL CENTER · PI WALTER J. CHAZIN, DAVID P. GIEDROC · 2013 to 2026
$8.2M
MEDICAL SCIENTIST TRAINING PROGRAMT32GM008620 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI GOLDIN, ALAN L · 1999 to 2023
$8.1M
MOLECULAR BIOPHYSICS TRAINING PROGRAM AT VANDERBILTT32GM008320 · NIGMS · VANDERBILT UNIVERSITY · PI CHAZIN, WALTER J. · 1989 to 2023
$7.9M
INTESTINAL VITAMIN H UPTAKE--MOLECULAR/CELL REGULATIONR01DK058057 · NIDDK · UNIVERSITY OF CALIFORNIA-IRVINE · PI SAID, HAMID M · 2000 to 2019
$6.8M
Immunology Research Training ProgramT32AI060573 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI PEARLMAN, ERIC · 2005 to 2020
$1.9M
Mucosal barrier function during Salmonella infectionR01AI083663 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI RAFFATELLU, MANUELA · 2010 to 2014
$1.9M
Development of siderophore-based vaccines against non-typhoidal Salmonella infectionR01AI114625 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NOLAN, ELIZABETH M, RAFFATELLU, MANUELA · 2015 to 2019
$1.9M
The role of the circadian clock during Salmonella infectionR21AI105374 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI RAFFATELLU, MANUELA, SASSONE-CORSI, PAOLO · 2014 to 2015
$424k
Metal withholding responses in the gut mucosaR21AI083619 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI RAFFATELLU, MANUELA · 2009 to 2010
$419k
Targeting iron acquisition in Salmonella with siderophore-based immunizationR21AI101784 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI NOLAN, ELIZABETH M, RAFFATELLU, MANUELA · 2014 to 2015
$418k
BLRD VA I01 BX002482NIAID NIH HHS P01 AI095208NIAID NIH HHS R01 AI083663NIAID NIH HHS R01 AI101171NIAID NIH HHS R01 AI114625NIAID NIH HHS R21 AI083619NIAID NIH HHS R21 AI099687NIAID NIH HHS R21 AI101784NIAID NIH HHS R21 AI105374NIAID NIH HHS R56 AI114625NIAID NIH HHS T32 AI060573NIDDK NIH HHS R01 DK058057NIGMS NIH HHS R25 GM055246NIGMS NIH HHS T32 GM008320NIGMS NIH HHS T32 GM008620
6 · The paper itself

Abstract

Neutrophils hinder bacterial growth by a variety of antimicrobial mechanisms, including the production of reactive oxygen species and the secretion of proteins that sequester nutrients essential to microbes. A major player in this process is calprotectin, a host protein that exerts antimicrobial activity by chelating zinc and manganese. Here we show that the intestinal pathogen Salmonella enterica serovar Typhimurium uses specialized metal transporters to evade calprotectin sequestration of manganese, allowing the bacteria to outcompete commensals and thrive in the inflamed gut. The pathogen's ability to acquire manganese in turn promotes function of SodA and KatN, enzymes that use the metal as a cofactor to detoxify reactive oxygen species. This manganese-dependent SodA activity allows the bacteria to evade neutrophil killing mediated by calprotectin and reactive oxygen species. Thus, manganese acquisition enables S. Typhimurium to overcome host antimicrobial defenses and support its competitive growth in the intestine.

Indexed as

AnimalsAnti-Bacterial AgentsAntioxidantsBacterial ProteinsChelating AgentsEscherichia coliGastroenteritisIntestinal MucosaIntestinesLeukocyte L1 Antigen ComplexManganeseMiceMice, Inbred C57BLNeutrophilsOxidative StressReactive Oxygen SpeciesAnti-Bacterial AgentsAntioxidantsBacterial ProteinsChelating AgentsLeukocyte L1 Antigen ComplexManganeseReactive Oxygen SpeciesZinc

Identifiers

PMID27281571
PMCPMC4901528
OpenAlexW2418650982

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.