ArticleInvestigative ophthalmology & visual science2016
Connexin 50 Regulates Surface Ball-and-Socket Structures and Fiber Cell Organization.
Article in Investigative ophthalmology & visual science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 29 citations in OpenAlex.
- The Human Lens Fiber Cytoskeletome Reveals Neuronal Signatures and the Presence of Chaperonins, Proteasome, Signaling, and Redox Regulators.Investigative ophthalmology & visual science · 2026Article
- The Double-Edged Effect of Connexins and Pannexins of Glial Cells in Central and Peripheral Nervous System After Nerve Injury.Molecular neurobiology · 2025Review
- Periaxin gene variants are linked to age-related cataracts in Cx46 deficient lenses.Communications biology · 2025Article
- Ankyrin-B is required for the establishment and maintenance of lens cytoarchitecture, mechanics and clarity.Journal of cell science · 2024Article
- Deficiency in glutathione peroxidase 4 (GPX4) results in abnormal lens development and newborn cataract.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Charged multivesicular body protein 4b forms complexes with gap junction proteins during lens fiber cell differentiation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- A tamoxifen-inducible Cre knock-in mouse for lens-specific gene manipulation.Experimental eye research · 2023Article
- Review
- Beyond the Channels: Adhesion Functions of Aquaporin 0 and Connexin 50 in Lens Development.Frontiers in cell and developmental biology · 2022Review
- Arvcf Dependent Adherens Junction Stability is Required to Prevent Age-Related Cortical Cataracts.Frontiers in cell and developmental biology · 2022Article
- Mechanical Stress Modulates Calcium-Activated-Chloride Currents in Differentiating Lens Cells.Frontiers in physiology · 2022Article
- Age-related changes of lens stiffness in wild-type and Cx46 knockout mice.Experimental eye research · 2021Article
- Biochemical and biomechanical characteristics of dystrophin-deficient mdxBiochimica et biophysica acta. Molecular basis of disease · 2021Article
- Connexin 50 and AQP0 are Essential in Maintaining Organization and Integrity of Lens Fibers.Investigative ophthalmology & visual science · 2019Article
- Calcium-Activated Chloride Channels in Newly Differentiating Mouse Lens Fiber Cells and Their Role in Volume Regulation.Investigative ophthalmology & visual science · 2019Article
- Proteome-transcriptome analysis and proteome remodeling in mouse lens epithelium and fibers.Experimental eye research · 2019Article
- A comprehensive spatial-temporal transcriptomic analysis of differentiating nascent mouse lens epithelial and fiber cells.Experimental eye research · 2018Article
- Induction of Immune Surveillance of the Dysmorphogenic Lens.Scientific reports · 2017Article
- N-cadherin regulates signaling mechanisms required for lens fiber cell elongation and lens morphogenesis.Developmental biology · 2017Article
- Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe roles of gap junction protein connexin 50 (Cx50) encoded by Gja8, during lens development are not fully understood. Connexin 50 knockout (KO) lenses have decreased proliferation of epithelial cells and altered fiber cell denucleation. We further investigated the mechanism for cellular defects in Cx50 KO (Gja8-/-) lenses.
methodsFiber cell morphology and subcellular distribution of various lens membrane/cytoskeleton proteins from wild-type and Cx50 KO mice were visualized by immunofluorescent staining and confocal microscopy.
resultsWe observed multiple morphological defects in the cortical fibers of Cx50 KO lenses, including abnormal fiber cell packing geometry, decreased F-actin enrichment at tricellular vertices, and disrupted ball-and-socket (BS) structures on the long sides of hexagonal fibers. Moreover, only small gap junction plaques consisting of Cx46 (α3 connexin) were detected in cortical fibers and the distributions of the BS-associated beta-dystroglycan and ZO-1 proteins were altered.
conclusionsConnexin 50 gap junctions are important for BS structure maturation and cortical fiber cell organization. Connexin 50-based gap junction plaques likely form structural domains with an array of membrane/cytoskeletal proteins to stabilize BS. Loss of Cx50-mediated coupling, BS disruption, and altered F-actin in Cx50 KO fibers, thereby contribute to the small lens and mild cataract phenotypes.
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