ArticlePloS one2016
Inherent Variability of Growth Media Impacts the Ability of Salmonella Typhimurium to Interact with Host Cells.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed.
- Stress Tolerance Assays for Salmonella enterica: Methods for Evaluating Heat, Acidity, and Osmotic Resistance.Methods in molecular biology (Clifton, N.J.) · 2027Article
- LuxS/AI-2 regulates phoP/phoQ by a non-canonical mechanism to enhance acid stress survival in Salmonella Typhimurium.PLoS pathogens · 2026Article
- How Bacterial Pathogens Coordinate Appetite with Virulence.Microbiology and molecular biology reviews : MMBR · 2023Review
- CsrA Positively and Directly Regulates the Expression of theMicrobiology spectrum · 2023Article
- Effect of Environmental Temperatures on Proteome Composition of Salmonella enterica Serovar Typhimurium.Molecular & cellular proteomics : MCP · 2022Article
- The expression of virulence genes increases membrane permeability and sensitivity to envelope stress in Salmonella Typhimurium.PLoS biology · 2022Article
- Reciprocally rewiring and repositioning the Integration Host Factor (IHF) subunit genes inMicrobial genomics · 2022Article
- The context matrix: Navigating biological complexity for advanced biodesign.Frontiers in bioengineering and biotechnology · 2022Article
- Genome-wide analysis of fitness-factors in uropathogenicMicrobial genomics · 2021Article
- An incoherent feedforward loop formed by SirA/BarA, HilE and HilD is involved in controlling the growth cost of virulence factor expression by Salmonella Typhimurium.PLoS pathogens · 2021Article
- Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC.Nature communications · 2021Article
- Characterization of Basal Transcriptomes Identifies Potential Metabolic and Virulence-Associated Adaptations Among Diverse NontyphoidalFrontiers in microbiology · 2021Article
- L-Arabinose Transport and Metabolism inFrontiers in cellular and infection microbiology · 2021Article
- Differential Packaging Into Outer Membrane Vesicles Upon Oxidative Stress Reveals a General Mechanism for Cargo Selectivity.Frontiers in microbiology · 2021Article
- Zinc, an unexpected integrator of metabolism?Microbial biotechnology · 2020Article
- Potential Plasticity of the Mannoprotein Repertoire Associated toMolecules (Basel, Switzerland) · 2020Article
- Reconciling high-throughput gene essentiality data with metabolic network reconstructions.PLoS computational biology · 2019Article
- Novel Imidazole and Methoxybenzylamine Growth Inhibitors Affecting Salmonella Cell Envelope Integrity and its Persistence in Chickens.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Efficient invasion of non-phagocytic cells, such as intestinal epithelial cells, by Salmonella Typhimurium is dependent on the Salmonella Pathogenicity Island 1 (SPI-1)-encoded Type Three Secretion System. The environmental cues involved in SPI-1 induction are not well understood. In vitro, various conditions are used to induce SPI-1 and the invasive phenotype. Although lysogeny broth (LB) is widely used, multiple formulations exist, and variation can arise due to intrinsic differences in complex components. Minimal media are also susceptible to variation. Still, the impact of these inconsistencies on Salmonella virulence gene expression has not been well studied. The goal of this project is to identify growth conditions in LB and minimal medium that affect SPI-1 induction in vitro using both whole population and single cell analysis. Here we show, using a fluorescent reporter of the SPI-1 gene prgH, that growth of Salmonella in LB yields variable induction. Deliberate modification of media components can influence the invasive profile. Finally, we demonstrate that changes in SPI-1 inducing conditions can affect the ability of Salmonella to replicate intracellularly. These data indicate that the specific media growth conditions impact how the bacteria interact with host cells.
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