ArticlePeerJ2016
Assessing Illumina technology for the high-throughput sequencing of bacteriophage genomes.
Article in PeerJ, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Who cites it
32 citing papers in PubMed.
- Same-day tagmentation PCR-based whole genome sequencing of bacteriophage genomes from a single plaque without DNA extraction.Scientific reports · 2026Article
- Computational prediction resolves thousands of homooligomeric phage protein structures.bioRxiv : the preprint server for biology · 2026Article
- Rolling outMicrobial genomics · 2026Article
- Population ecology and biogeochemical implications of ssDNA and dsDNA viruses along a permafrost thaw gradient.Nature communications · 2025Article
- Characterization, Genomic Analysis and Application of Five Lytic Phages Against Carbapenem-ResistantMicroorganisms · 2025Article
- Short-read and Long-read PCR-Free Sequencing of Bacteriophages Using Ultra-Low Starting DNA Input.Journal of biomolecular techniques : JBT · 2025Article
- Characterization and application of a novelCurrent research in food science · 2025Article
- Diverse phage communities are maintained stably on a clonal bacterial host.Science (New York, N.Y.) · 2024Article
- The long and short of it: benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies.Microbial genomics · 2024Article
- Coordinated transcriptional response to environmental stress by a Synechococcus virus.The ISME journal · 2024Article
- Genome Sequence and Characterization of Coliphage Môr Ffagbaw.PHAGE (New Rochelle, N.Y.) · 2023Article
- Genomic analysis of Anderson typing phages of Salmonella Typhimrium: towards understanding the basis of bacteria-phage interaction.Scientific reports · 2023Article
- Crop management shapes the diversity and activity of DNA and RNA viruses in the rhizosphere.Microbiome · 2022Article
- INfrastructure for a PHAge REference Database: Identification of Large-Scale Biases in the Current Collection of Cultured Phage Genomes.PHAGE (New Rochelle, N.Y.) · 2021Article
- Phage Annotation Guide: Guidelines for Assembly and High-Quality Annotation.PHAGE (New Rochelle, N.Y.) · 2021Article
- Phage Genome Annotation: Where to Begin and End.PHAGE (New Rochelle, N.Y.) · 2021Article
- A new family of globally distributed lytic roseophages with unusual deoxythymidine to deoxyuridine substitution.Current biology : CB · 2021Article
- Bacteriophages in Biological Wastewater Treatment Systems: Occurrence, Characterization, and Function.Frontiers in microbiology · 2021Review
- Genome Sequence and Characterization of Coliphage vB_Eco_SLUR29.PHAGE (New Rochelle, N.Y.) · 2020Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Bacteriophages are the most abundant biological entities on the planet, playing crucial roles in the shaping of bacterial populations. Phages have smaller genomes than their bacterial hosts, yet there are currently fewer fully sequenced phage than bacterial genomes. We assessed the suitability of Illumina technology for high-throughput sequencing and subsequent assembly of phage genomes. In silico datasets reveal that 30× coverage is sufficient to correctly assemble the complete genome of ~98.5% of known phages, with experimental data confirming that the majority of phage genomes can be assembled at 30× coverage. Furthermore, in silico data demonstrate it is possible to co-sequence multiple phages from different hosts, without introducing assembly errors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.