Evidence map›Paper›PMID 27280007›Full record

ArticleElectronic physician2016

Molecular typing of uropathogenic E. coli strains by the ERIC-PCR method.

Maryam Afkhami Ardakani, Reza Ranjbar

Abstract read
In one paragraph

Article in Electronic physician, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Article
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  7. Molecular characterization of ESBL-producingFrontiers in cellular and infection microbiology · 2026
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  16. Observational
  17. Detection of Antibiotic Resistance Determinants and Their Transmissibility among Clinically Isolated Carbapenem-Resistant Escherichia coli from South India.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2018
    Article
  18. Article
  19. Genotyping of virulentVeterinary world · 2017
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Maryam Afkhami ArdakaniM.Sc. of Microbiology, Department of Microbiology, Faculty of Advanced Science & Technology, pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
Reza RanjbarPh.D. of Medical Bacteriology, Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionEscherichia coli (E. coli) is the most common cause of urinary infections in hospitals. The aim of this study was to evaluate the ERIC-PCR method for molecular typing of uropathogenic E. coli strains isolated from hospitalized patients.

methodsIn a cross sectional study, 98 E. coli samples were collected from urine samples taken from patients admitted to Baqiyatallah Hospital from June 2014 to January 2015. The disk agar diffusion method was used to determine antibiotic sensitivity. DNA proliferation based on repetitive intergenic consensus was used to classify the E. coli strains. The products of proliferation were electrophoresed on 1.5% agarose gel, and their dendrograms were drawn. The data were analyzed by online Insillico software.

resultsThe method used in this research proliferated numerous bands (4-17 bands), ranging from 100 to 3000 base pairs. The detected strains were classified into six clusters (E1-E6) with 70% similarity between them.

conclusionIn this study, uropathogenic E. coli strains belonged to different genotypic clusters. It was found that ERIC-PCR had good differentiation power for molecular typing of uropathogenic E. coli strains isolated from the patients in the study.

Indexed as

ERIC-PCRmolecular typingurinary tract infectionuropathogenic E. coli

Identifiers

PMID27280007
PMCPMC4886573

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