ArticlePlant methods2016
RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation.
Article in Plant methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Rapid detection and quantification of sweet potato storage roots using ground penetrating radar.Plant phenomics (Washington, D.C.) · 2026Article
- Advances in root phenotyping: high-throughput imaging, computational tools, and integrative approaches for crop improvement.Journal of experimental botany · 2026Review
- GrowScreen-Rhizo 3 - automated large-scale high throughput greenhouse phenotyping of plant root and shoot development.Plant phenomics (Washington, D.C.) · 2026Article
- Integrating multi-omics approaches to shape legume root system architecture under drought stress: a comprehensive review.Frontiers in plant science · 2026Review
- A novel P indicator to evaluate bread wheat (Triticum aestivum) genotypes to identify tolerance to phosphorus deficiency based on two distinct root phenotyping platforms.Annals of botany · 2025Article
- Molecular Network Analysis in Model and Non-Model Legumes: Challenges in Omics Data Interpretation Across Species, with a Focus onPlants (Basel, Switzerland) · 2025Review
- Integrating 3D imaging, GWAS, and single-cell transcriptome approaches to elucidate root system architecture in Populus.Plant physiology · 2025Article
- Mixing varieties mitigates early root competition in wheat under water and nutrient limitation.Journal of experimental botany · 2025Article
- 2023: a soil odyssey-HeAted soiL-Monoliths (HAL-Ms) to examine the effect of heat emission from HVDC underground cables on plant growth.Plant methods · 2024Article
- Comparative Analysis of Water Stress Regimes in Avocado Plants during the Early Development Stage.Plants (Basel, Switzerland) · 2024Article
- Non-destructive real-time monitoring of underground root development with distributed fiber optic sensing.Plant methods · 2024Article
- Plant Physiological Analysis to Overcome Limitations to Plant Phenotyping.Plants (Basel, Switzerland) · 2023Review
- Rhizosphere frame system enables nondestructive live-imaging of legume-rhizobium interactions in the soil.Journal of plant research · 2023Article
- Root plasticity: an effective selection technique for identification of drought tolerant maize (Zea mays L.) inbred lines.Scientific reports · 2023Article
- Quantification of the three-dimensional root system architecture using an automated rotating imaging system.Plant methods · 2023Article
- Integrated phenotyping of root and shoot growth dynamics in maize reveals specific interaction patterns in inbreds and hybrids and in response to drought.Frontiers in plant science · 2023Article
- The rhizodynamics robot: Automated imaging system for studying long-term dynamic root growth.PloS one · 2023Article
- Crop root system plasticity for improved yields in saline soils.Frontiers in plant science · 2023Review
- High-throughput and automatic structural and developmental root phenotyping on Arabidopsis seedlings.Plant methods · 2022Article
- Pea Breeding for Resistance to Rhizospheric Pathogens.Plants (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
20 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundIn order to maintain high yields while saving water and preserving non-renewable resources and thus limiting the use of chemical fertilizer, it is crucial to select plants with more efficient root systems. This could be achieved through an optimization of both root architecture and root uptake ability and/or through the improvement of positive plant interactions with microorganisms in the rhizosphere. The development of devices suitable for high-throughput phenotyping of root structures remains a major bottleneck.
resultsRhizotrons suitable for plant growth in controlled conditions and non-invasive image acquisition of plant shoot and root systems (RhizoTubes) are described. These RhizoTubes allow growing one to six plants simultaneously, having a maximum height of 1.1 m, up to 8 weeks, depending on plant species. Both shoot and root compartment can be imaged automatically and non-destructively throughout the experiment thanks to an imaging cabin (RhizoCab). RhizoCab contains robots and imaging equipment for obtaining high-resolution pictures of plant roots. Using this versatile experimental setup, we illustrate how some morphometric root traits can be determined for various species including model (Medicago truncatula), crops (Pisum sativum, Brassica napus, Vitis vinifera, Triticum aestivum) and weed (Vulpia myuros) species grown under non-limiting conditions or submitted to various abiotic and biotic constraints. The measurement of the root phenotypic traits using this system was compared to that obtained using "classic" growth conditions in pots.
conclusionsThis integrated system, to include 1200 Rhizotubes, will allow high-throughput phenotyping of plant shoots and roots under various abiotic and biotic environmental conditions. Our system allows an easy visualization or extraction of roots and measurement of root traits for high-throughput or kinetic analyses. The utility of this system for studying root system architecture will greatly facilitate the identification of genetic and environmental determinants of key root traits involved in crop responses to stresses, including interactions with soil microorganisms.
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