ArticleScientific reports2016
Mass spectrometry analysis and transcriptome sequencing reveal glowing squid crystal proteins are in the same superfamily as firefly luciferase.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Genetic diversity and trait associations in biofortified short-duration sweet potato (Ipomoea Batatas L.) genotypes.BMC plant biology · 2025Article
- Biochemical basis of endogenous bioluminescent springtail Lobella sauteri (Collembola).Biology open · 2025Article
- A New Method for Albuminuria Measurement Using a Specific Reaction between Albumin and the Luciferin of the Firefly SquidInternational journal of molecular sciences · 2022Article
- Violet bioluminescent Polycirrus sp. (Annelida: Terebelliformia) discovered in the shallow coastal waters of the Noto Peninsula in Japan.Scientific reports · 2021Article
- Genomic and Transcriptomic Analyses of Bioluminescence Genes in the Enope Squid Watasenia scintillans.Marine biotechnology (New York, N.Y.) · 2020Article
- Terrestrial and marine bioluminescent organisms from the Indian subcontinent: a review.Environmental monitoring and assessment · 2020Review
- Discovery of Novel Crustacean and Cephalopod Flaviviruses: Insights into the Evolution and Circulation of Flaviviruses between Marine Invertebrate and Vertebrate Hosts.Journal of virology · 2019Article
- Advances in bioluminescence imaging: new probes from old recipes.Current opinion in chemical biology · 2018Review
- New Zealand glowworm (Arachnocampa luminosa) bioluminescence is produced by a firefly-like luciferase but an entirely new luciferin.Scientific reports · 2018Article
- A puzzling homology: a brittle star using a putative cnidarian-type luciferase for bioluminescence.Open biology · 2017Article
- Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Japanese firefly squid Hotaru-ika (Watasenia scintillans) produces intense blue light from photophores at the tips of two arms. These photophores are densely packed with protein microcrystals that catalyse the bioluminescent reaction using ATP and the substrate coelenterazine disulfate. The squid is the only organism known to produce light using protein crystals. We extracted microcrystals from arm tip photophores and identified the constituent proteins using mass spectrometry and transcriptome libraries prepared from arm tip tissue. The crystals contain three proteins, wsluc1-3, all members of the ANL superfamily of adenylating enzymes. They share 19 to 21% sequence identity with firefly luciferases, which produce light using ATP and the unrelated firefly luciferin substrate. We propose that wsluc1-3 form a complex that crystallises inside the squid photophores, and that in the crystal one or more of the proteins catalyses the production of light using coelenterazine disulfate and ATP. These results suggest that ANL superfamily enzymes have independently evolved in distant species to produce light using unrelated substrates.
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