ArticleScientific reports2016
Efficient production of acetoin in Saccharomyces cerevisiae by disruption of 2,3-butanediol dehydrogenase and expression of NADH oxidase.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 87 citations in OpenAlex.
- Rose powder modulates the quality and volatile flavor profile of steamed buns containing red yeast rice powder.Food chemistry: X · 2026Article
- Metabolic and Synthetic Biology Strategies for Enhancing Single-Cell Protein Production in Saccharomyces cerevisiae.Biotechnology journal · 2026Review
- Exploring ethyl methane sulphonate induced, L-cysteine HCl resistant Bacillus subtilis putative mutant and its immobilization for enhanced acetoin synthesis.Archives of microbiology · 2026Article
- Regeneration of cofactor NAD(P)Frontiers in bioengineering and biotechnology · 2025Review
- Flavour chemistry and metabolic engineering of microbial synthetic pyrazines: decoding the path to green manufacturing of food aroma components.Current research in food science · 2025Review
- HyperpolarizedMolecules (Basel, Switzerland) · 2024Article
- Roles of flavoprotein oxidase and the exogenous heme- and quinone-dependent respiratory chain in lactic acid bacteria.Bioscience of microbiota, food and health · 2024Review
- Transcriptomic and metabolomic correlation analysis: effect of initial SOFrontiers in microbiology · 2024Article
- Snapshot of the Probiotic Potential ofFoods (Basel, Switzerland) · 2023Article
- Efficient production of acetoin from lactate by engineered Escherichia coli whole-cell biocatalyst.Applied microbiology and biotechnology · 2023Article
- Metabolic Engineering ofMicroorganisms · 2023Article
- Acetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in Bacillus subtilis.Biotechnology for biofuels and bioproducts · 2022Article
- Engineering acetyl-CoA supply and ERG9 repression to enhance mevalonate production in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2021Article
- Efficient Conversion of Glycerol to Ethanol by an Engineered Saccharomyces cerevisiae Strain.Applied and environmental microbiology · 2021Article
- Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae.Biotechnology for biofuels · 2021Article
- Optimization of process variables for acetoin production in a bioreactor using Taguchi orthogonal array design.Heliyon · 2020Article
- Engineering central pathways for industrial-level (3R)-acetoin biosynthesis in Corynebacterium glutamicum.Microbial cell factories · 2020Article
- Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2020Article
- Increasing Yield of 2,3,5,6-Tetramethylpyrazine in Baijiu ThroughFrontiers in microbiology · 2020Article
- Monitoring type 2 diabetes from volatile faecal metabolome in Cushing's syndrome and single Afmid mouse models via a longitudinal study.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acetoin is widely used in food and cosmetic industry as taste and fragrance enhancer. For acetoin production in this study, Saccharomyces cerevisiae JHY605 was used as a host strain, where the production of ethanol and glycerol was largely eliminated by deleting five alcohol dehydrogenase genes (ADH1, ADH2, ADH3, ADH4, and ADH5) and two glycerol 3-phosphate dehydrogenase genes (GPD1 and GPD2). To improve acetoin production, acetoin biosynthetic genes from Bacillus subtilis encoding α-acetolactate synthase (AlsS) and α-acetolactate decarboxylase (AlsD) were overexpressed, and BDH1 encoding butanediol dehydrogenase, which converts acetoin to 2,3-butanediol, was deleted. Furthermore, by NAD(+) regeneration through overexpression of water-forming NADH oxidase (NoxE) from Lactococcus lactis, the cofactor imbalance generated during the acetoin production from glucose was successfully relieved. As a result, in fed-batch fermentation, the engineered strain JHY617-SDN produced 100.1 g/L acetoin with a yield of 0.44 g/g glucose.
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