ArticleBiophysical journal2016
Actomyosin Cortical Mechanical Properties in Nonadherent Cells Determined by Atomic Force Microscopy.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 129 citations in OpenAlex.
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- Data-driven analysis for the evaluation of cortical mechanics of non-adherent cells.Scientific reports · 2025Article
- Nucleating amoeboid cancer cell motility with Diaphanous related formins.Cytoskeleton (Hoboken, N.J.) · 2025Review
- Proteolysis-free amoeboid migration of melanoma cells through crowded environments via bleb-driven worrying.Developmental cell · 2024Article
- Deformation under flow and morphological recovery of cancer cells.Lab on a chip · 2024Article
- The structure and mechanics of the cell cortex depend on the location and adhesion state.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Cell-intrinsic mechanical regulation of plasma membrane accumulation at the cytokinetic furrow.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Mechanical properties of human tumour tissues and their implications for cancer development.Nature reviews. Physics · 2024Article
- Cell intrinsic mechanical regulation of plasma membrane accumulation at the cytokinetic furrow.bioRxiv : the preprint server for biology · 2024Article
- Monitoring the mass, eigenfrequency, and quality factor of mammalian cells.Nature communications · 2024Article
- Quantifying both viscoelasticity and surface tension: Why sharp tips overestimate cell stiffness.Biophysical journal · 2024Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The organization of filamentous actin and myosin II molecular motor contractility is known to modify the mechanical properties of the cell cortical actomyosin cytoskeleton. Here we describe a novel method, to our knowledge, for using force spectroscopy approach curves with tipless cantilevers to determine the actomyosin cortical tension, elastic modulus, and intracellular pressure of nonadherent cells. We validated the method by measuring the surface tension of water in oil microdrops deposited on a glass surface. We extracted an average tension of T ∼ 20.25 nN/μm, which agrees with macroscopic experimental methods. We then measured cortical mechanical properties in nonadherent human foreskin fibroblasts and THP-1 human monocytes before and after pharmacological perturbations of actomyosin activity. Our results show that myosin II activity and actin polymerization increase cortex tension and intracellular pressure, whereas branched actin networks decreased them. Interestingly, myosin II activity stiffens the cortex and branched actin networks soften it, but actin polymerization has no effect on cortex stiffness. Our method is capable of detecting changes in cell mechanical properties in response to perturbations of the cytoskeleton, allowing characterization with physically relevant parameters. Altogether, this simple method should be of broad application for deciphering the molecular regulation of cell cortical mechanical properties.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.