Evidence map›Paper›PMID 27276261›Full record

ArticleBiophysical journal2016

Effects of Lipid Tethering in Extremophile-Inspired Membranes on H(+)/OH(-) Flux at Room Temperature.

Thomas B H Schroeder, Geoffray Leriche, Takaoki Koyanagi, Mitchell A Johnson, Kathryn N Haengel, Olivia M Eggenberger, Claire L Wang, Young Hun Kim, Karthik Diraviyam, David Sept and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Thomas B H SchroederDepartment of Chemical Engineering, University of Michigan, Ann Arbor, Michigan; Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland.
Geoffray LericheDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, California.
Takaoki KoyanagiDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, California.
Mitchell A JohnsonDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan.
Kathryn N HaengelDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan.
Olivia M EggenbergerAdolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan.
Claire L WangDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan.
Young Hun KimDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, California.
Karthik DiraviyamDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan.
David SeptDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan.
Jerry YangDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, California.
Michael MayerAdolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan. Electronic address: michael.mayer@unifr.ch.
University of Michigan–Ann Arbor · USUniversity of California, San Diego · USAdolphe Merkle Institute · CH

Funding

Cellular Biotechnology Training Program (CBTP)T32GM008353 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SCHWENDEMAN, ANNA · 1991 to 2021
$9.3M
Cellular Biotechnology Training Program (CBTP) - Years 31-35T32GM145304 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Guizhi Zhu · 2022 to 2026
$2.6M
NIGMS NIH HHS T32 GM008353NIGMS NIH HHS T32 GM145304
6 · The paper itself

Abstract

This work explores the proton/hydroxide permeability (PH+/OH-) of membranes that were made of synthetic extremophile-inspired phospholipids with systematically varied structural elements. A fluorescence-based permeability assay was optimized to determine the effects on the PH+/OH- through liposome membranes with variations in the following lipid attributes: transmembrane tethering, tether length, and the presence of isoprenoid methyl groups on one or both lipid tails. All permeability assays were performed in the presence of a low concentration of valinomycin (10 nM) to prevent buildup of a membrane potential without artificially increasing the measured PH+/OH-. Surprisingly, the presence of a transmembrane tether did not impact PH+/OH- at room temperature. Among tethered lipid monolayers, PH+/OH- increased with increasing tether length if the number of carbons in the untethered acyl tail was constant. Untethered lipids with two isoprenoid methyl tails led to lower PH+/OH- values than lipids with only one or no isoprenoid tails. Molecular dynamics simulations revealed a strong positive correlation between the probability of observing water molecules in the hydrophobic core of these lipid membranes and their proton permeability. We propose that water penetration as revealed by molecular dynamics may provide a general strategy for predicting proton permeability through various lipid membranes without the need for experimentation.

Indexed as

ProtonsArchaeaBiomimetic MaterialsFluorescent DyesHydrogen-Ion ConcentrationHydrophobic and Hydrophilic InteractionsHydroxidesIonophoresLiposomesMembrane LipidsMembrane PotentialsMethacrylatesMicroscopy, Atomic ForceMolecular Dynamics SimulationPermeabilityUnilamellar LiposomesFluorescent Dyeshydroxide ionHydroxidesIonophoresLiposomesMembrane LipidsMethacrylatesProtonsTemphaseUnilamellar LiposomesValinomycinWater

Identifiers

PMID27276261
PMCPMC4906265
OpenAlexW2419293019

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.