Evidence map›Paper›PMID 27275481›Full record

ArticleAnnals of translational medicine2016

Use of GeneXpert Mycobacterium tuberculosis/rifampicin for rapid detection of rifampicin resistant Mycobacterium tuberculosis strains of clinically suspected multi-drug resistance tuberculosis cases.

Kheira Guenaoui, Noria Harir, Aissa Ouardi, Soumia Zeggai, Feriel Sellam, Farid Bekri, Sakina Cherif Touil

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of translational medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 5 pooled it
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 5 syntheses or guidelines pooled it, 37 citations in OpenAlex.

  1. Pooled it
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  6. Article
  7. Article
  8. Article
  9. Review
  10. Rifampicin resistance inNew microbes and new infections · 2021
    Article
  11. Observational
  12. Clinical risk factors associated with multidrug-resistant tuberculosis (MDR-TB) in Mali.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2019
    Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Kheira Guenaoui1 Laboratoty of molecular microbiology, proteomics and Health, Department of Biology, Djillali Liabes University of Sidi bel Abbes, Sidi Bel Abbès, Algeria ; 2 Department of Pneumophtisiology (point B), CHU Oran, Oran, Algeria ; 3 Department of Biological Hematology, Tenon Hospital, Paris, France.
Noria Harir1 Laboratoty of molecular microbiology, proteomics and Health, Department of Biology, Djillali Liabes University of Sidi bel Abbes, Sidi Bel Abbès, Algeria ; 2 Department of Pneumophtisiology (point B), CHU Oran, Oran, Algeria ; 3 Department of Biological Hematology, Tenon Hospital, Paris, France.
Aissa Ouardi1 Laboratoty of molecular microbiology, proteomics and Health, Department of Biology, Djillali Liabes University of Sidi bel Abbes, Sidi Bel Abbès, Algeria ; 2 Department of Pneumophtisiology (point B), CHU Oran, Oran, Algeria ; 3 Department of Biological Hematology, Tenon Hospital, Paris, France.
Soumia Zeggai1 Laboratoty of molecular microbiology, proteomics and Health, Department of Biology, Djillali Liabes University of Sidi bel Abbes, Sidi Bel Abbès, Algeria ; 2 Department of Pneumophtisiology (point B), CHU Oran, Oran, Algeria ; 3 Department of Biological Hematology, Tenon Hospital, Paris, France.
Feriel Sellam1 Laboratoty of molecular microbiology, proteomics and Health, Department of Biology, Djillali Liabes University of Sidi bel Abbes, Sidi Bel Abbès, Algeria ; 2 Department of Pneumophtisiology (point B), CHU Oran, Oran, Algeria ; 3 Department of Biological Hematology, Tenon Hospital, Paris, France.
Farid Bekri1 Laboratoty of molecular microbiology, proteomics and Health, Department of Biology, Djillali Liabes University of Sidi bel Abbes, Sidi Bel Abbès, Algeria ; 2 Department of Pneumophtisiology (point B), CHU Oran, Oran, Algeria ; 3 Department of Biological Hematology, Tenon Hospital, Paris, France.
Sakina Cherif Touil1 Laboratoty of molecular microbiology, proteomics and Health, Department of Biology, Djillali Liabes University of Sidi bel Abbes, Sidi Bel Abbès, Algeria ; 2 Department of Pneumophtisiology (point B), CHU Oran, Oran, Algeria ; 3 Department of Biological Hematology, Tenon Hospital, Paris, France.
Université Djilali de Sidi Bel Abbès · DZHôpital Tenon · FRSorbonne Université · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMulti-drug resistance (MDR) TB is defined as tuberculosis (TB) disease caused by a strain of Mycobacterium tuberculosis (MTB) that was resistant to at least isoniazid and rifampicin (RIF). Emerging Multidrug-Resistant TB is one of the major concerns of health policy and rapid detection of M. tuberculosis and detection of RIF resistance in infected patients are essential for disease management. The aim of this study was to evaluate patterns of RIF resistance in cases of sputum positive pulmonary TB by using GeneXpert MTB/RIF and comparing between phenotypic and genotypic testing of RIF resistance in MTB strains of clinically suspected MDR-TB isolated cases in western Algeria.

methodsIn this study 50 sputum positive cases of pulmonary TB who were potential MDR suspect were included. Their sputum samples were collected and subjected to sputum smear microscopy, culture and conventional MTB/RIF test followed by GeneXpert MTB/RIF assay.

resultsOf total 50 cases included in this study, MTB was detected in all patients (100%) by GeneXpert MTB/RIF. However, RIF's resistance was detected in only 21 cases (42%) by GeneXpert MTB/RIF. All RIF resistant strains detected by GeneXpert MTB/RIF were phenotypically confirmed as MDR strains. 42.85% of cases were retreatment failure cases, retreatment cases smear positive at 4 months were 23.82%. While 19.05% of cases were retreatment cases smear positive at diagnosis, and 14.28% patient had history of contact with MDR-TB. Sensitivity, specificity, positive predictive value and negative predictive value of Xpert MTB/RIF to detect RIF resistance in comparison to conventional phenotypic drug susceptibility technique were found equal to the rates of 100%, 100%, 100% and 100%, respectively.

conclusionsGeneXpert MTB/RIF assay is efficient and reliable technique for the rapid diagnostic of TB. It's simplicity, high sensitivity and specificity for RIF resistance detection make this technique a very attractive tool for diagnostic of MTB and RIF resistance in MDR cases.

Indexed as

GeneXpert MTB/RIFmulti-drug resistance (MDR)Mycobacterium tuberculosis (MTB)rifampicin resistance

Identifiers

PMID27275481
PMCPMC4876276
OpenAlexW2399168760

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.