Evidence map›Paper›PMID 27275421›Full record

ArticleInternational journal of ophthalmology2016

Real-time polymerase chain reaction for the diagnosis of necrotizing herpes stromal keratitis.

Jun-Xin Ma, Lin-Nong Wang, Ru-Xia Zhou, Yang Yu, Tong-Xin Du

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of ophthalmology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. A study on childhood microbial keratitis in South India.Indian journal of ophthalmology · 2023
    Article
  4. Article
  5. Review
  6. Effectiveness of 16S ribosomal DNA real-time PCR and sequencing for diagnosing bacterial keratitis.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2020
    Article
  7. Article
  8. Article
  9. Commentary: Herpes keratitis: A diagnostic challenge.Indian journal of ophthalmology · 2019
    Article
  10. The evaluation of diagnostic efficiency for stromal herpes simplex keratitis by the combination of tear HSV-sIgA and HSV-DNA.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jun-Xin MaDepartment of Ophthalmology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, Jiangsu Province, China.
Lin-Nong WangDepartment of Ophthalmology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, Jiangsu Province, China.
Ru-Xia ZhouDepartment of Ophthalmology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, Jiangsu Province, China.
Yang YuDepartment of Clinical Nuclear Medicine Center, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, Jiangsu Province, China.
Tong-Xin DuDepartment of Clinical Nuclear Medicine Center, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, Jiangsu Province, China.
Nanjing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo design, optimize and validate a rapid, internally controlled real-time polymerase chain reaction (RT-PCR) test for herpes simplex virus (HSV) in the diagnosis of necrotizing herpes stromal keratitis.

methodsTears alone or together with corneal epithelium scrapings from 30 patients (30 eyes) suspected of necrotizing herpes stromal keratitis were tested for HSV DNA by RT-PCR. The samples were collected during the first visit and then on the subsequent 7, 14, 28, 42, and 56d. The symptoms of the patients were scored before treatment to determine the correlation between HSV concentration in the corneal epithelium scrapings and clinical scores.

resultsThe positive rate (46.4%) in the corneal epithelium group before the therapy was significantly higher than that (13.3%) in the tears group (P=0.006). There were 13 positive HSV patients before the therapy, the concentration of HSV DNA in corneal epithelium scrapings group was significantly higher than that in the tears group (paired t-test, P=0.0397). Multilevel mixed-effects model analysis showed that the difference between the corneal epithelium scrapings group and the tears group was statistically significant (P=0.0049). The Spearman rank correlation analysis indicated a positive correlation between the HSV concentration in the corneal epithelium scrapings and clinical scores before the treatment (r=0.844, P<0.0001).

conclusionRT-PCR appears to be a powerful molecular tool for the diagnosis of necrotizing herpes stromal keratitis.

Indexed as

corneal epithelium scrapingsnecrotizing herpes stromal keratitisreal-time polymerase chain reactiontears

Identifiers

PMID27275421
PMCPMC4886885
OpenAlexW2406425339

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.