Evidence map›Paper›PMID 27275093›Full record

ArticleWorld journal of gastroenterology2016

CdSe/ZnS quantum dots induce photodynamic effects and cytotoxicity in pancreatic cancer cells.

Si-Jia He, Jia Cao, Yong-Sheng Li, Jia-Chun Yang, Min Zhou, Chun-Ying Qu, Yi Zhang, Feng Shen, Ying Chen, Ming-Ming Li and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 41 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. Review
  8. Article
  9. Recent advances in photodynamic therapy for cancer and infectious diseases.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2019
    Review
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Si-Jia HeSi-Jia He, Jia Cao, Jia-Chun Yang, Min Zhou, Chun-Ying Qu, Yi Zhang, Feng Shen, Ying Chen, Ming-Ming Li, Lei-Ming Xu, Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
Jia CaoSi-Jia He, Jia Cao, Jia-Chun Yang, Min Zhou, Chun-Ying Qu, Yi Zhang, Feng Shen, Ying Chen, Ming-Ming Li, Lei-Ming Xu, Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
Yong-Sheng LiSi-Jia He, Jia Cao, Jia-Chun Yang, Min Zhou, Chun-Ying Qu, Yi Zhang, Feng Shen, Ying Chen, Ming-Ming Li, Lei-Ming Xu, Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
Jia-Chun YangSi-Jia He, Jia Cao, Jia-Chun Yang, Min Zhou, Chun-Ying Qu, Yi Zhang, Feng Shen, Ying Chen, Ming-Ming Li, Lei-Ming Xu, Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
Min ZhouSi-Jia He, Jia Cao, Jia-Chun Yang, Min Zhou, Chun-Ying Qu, Yi Zhang, Feng Shen, Ying Chen, Ming-Ming Li, Lei-Ming Xu, Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
Chun-Ying QuSi-Jia He, Jia Cao, Jia-Chun Yang, Min Zhou, Chun-Ying Qu, Yi Zhang, Feng Shen, Ying Chen, Ming-Ming Li, Lei-Ming Xu, Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
Yi ZhangSi-Jia He, Jia Cao, Jia-Chun Yang, Min Zhou, Chun-Ying Qu, Yi Zhang, Feng Shen, Ying Chen, Ming-Ming Li, Lei-Ming Xu, Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
Feng ShenSi-Jia He, Jia Cao, Jia-Chun Yang, Min Zhou, Chun-Ying Qu, Yi Zhang, Feng Shen, Ying Chen, Ming-Ming Li, Lei-Ming Xu, Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
Ying ChenSi-Jia He, Jia Cao, Jia-Chun Yang, Min Zhou, Chun-Ying Qu, Yi Zhang, Feng Shen, Ying Chen, Ming-Ming Li, Lei-Ming Xu, Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
Ming-Ming LiSi-Jia He, Jia Cao, Jia-Chun Yang, Min Zhou, Chun-Ying Qu, Yi Zhang, Feng Shen, Ying Chen, Ming-Ming Li, Lei-Ming Xu, Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
Lei-Ming XuSi-Jia He, Jia Cao, Jia-Chun Yang, Min Zhou, Chun-Ying Qu, Yi Zhang, Feng Shen, Ying Chen, Ming-Ming Li, Lei-Ming Xu, Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
XinHua Hospital · CNShanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo investigate the photodynamic effect of CdSe/ZnS quantum dots (QDs) on pancreatic cancer cells and elucidate the probable mechanisms.

methodsThe pancreatic cancer cell line SW1990 was treated with different concentrations of CdSe/ZnS QDs (0, 0.5, 1.0, 1.5, 2.0, 2.5 μmol/L), with or without illumination. The viability of SW1990 cells was tested using the Cell Counting Kit-8 (CCK-8) assay. The ultrastructural changes of SW1990 cells were observed by transmission electron microscopy. Apoptosis was detected by nuclear staining and flow cytometry (FCM). Reactive oxygen species (ROS) were measured by dichlorofluorescein diacetate via fluorescence microscopy. Expression of Bax, Bcl-2 and caspase-3 was measured by real-time polymerase chain reaction (PCR) and protein immunoblotting 24 h after SW1990 cells were treated with CdSe/ZnS QDs and illuminated.

resultsThe CCK-8 assay results showed that both CdSe/ZnS QDs with and without illumination suppressed SW1990 cell proliferation. Cell viability was significantly lower when illuminated or with a longer incubation time and a higher light dose. CdSe/ZnS QDs with illumination caused ultrastructural changes in SW1990 cells, such as organelle degeneration and chromatin condensation and aggregation at the periphery of the nucleus. Fluorescence microscopy and FCM showed that CdSe/ZnS QDs (1.5 μmol/L) with illumination increased SW1990 cell apoptosis (53.2%) and ROS generation compared with no illumination. Real-time PCR showed that expression of Bax and caspase-3 was upregulated and Bcl-2 was downregulated. Immunoblotting results were consistent with real-time PCR results. Inhibition of ROS and apoptosis both attenuated QD-photodynamic-therapy-induced cell death.

conclusionCdSe/ZnS QDs can be used as a photosensitizer to inhibit SW1990 cell proliferation through ROS generation and apoptotic protein expression regulation.

Indexed as

PhotochemotherapyQuantum DotsApoptosisApoptosis Regulatory ProteinsCadmium CompoundsCell Line, TumorCell ProliferationCell SurvivalDose-Response Relationship, DrugFlow CytometryGene Expression Regulation, NeoplasticHumansMicroscopy, Electron, TransmissionMicroscopy, FluorescenceOxidative StressPancreatic NeoplasmsApoptosis Regulatory ProteinsCadmium Compoundscadmium selenidePhotosensitizing AgentsReactive Oxygen SpeciesRNA, MessengerSelenium CompoundsSulfidesZinc Compoundszinc sulfideApoptosisPancreatic cancerPhotodynamic therapyQuantum dotsReactive oxygen species

Identifiers

PMID27275093
PMCPMC4886376
OpenAlexW2419159726

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.