ArticleJournal of physics D: Applied physics2016
Spot size variation FCS in simulations of the 2D Ising model.
Article in Journal of physics D: Applied physics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Cross-Correlation Increases Sampling in Diffusion-Based Super-Resolution Optical Fluctuation Imaging.Chemical & biomedical imaging · 2024Article
- Quantifying membrane binding and diffusion with fluorescence correlation spectroscopy diffusion laws.Biophysical journal · 2023Article
- Synergistic factors control kinase-phosphatase organization in B-cells engaged with supported bilayers.Molecular biology of the cell · 2020Article
- Anomalous Diffusion in Inverted Variable-Lengthscale Fluorescence Correlation Spectroscopy.Biophysical journal · 2019Article
- Dynamic pattern generation in cell membranes: Current insights into membrane organization.Biochimica et biophysica acta. Biomembranes · 2018Review
- FSCS Reveals the Complexity of Lipid Domain Dynamics in the Plasma Membrane of Live Cells.Biophysical journal · 2018Article
- The Continuing Mystery of Lipid Rafts.Journal of molecular biology · 2016Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Spot variation fluorescence correlation spectroscopy (svFCS) was developed to study the movement and organization of single molecules in plasma membranes. This experimental technique varies the size of an illumination area while measuring correlations in time using standard fluorescence correlation methods. Frequently, this data is interpreted using the assumption that correlation measurements reflect the dynamics of single molecule motions, and not motions of the average composition. Here, we explore how svFCS measurements report on the dynamics of components diffusing within simulations of a 2D Ising model with a conserved order parameter. Simulated correlation functions report on both the fast dynamics of single component mobility and the slower dynamics of the average composition. Over a range of simulation conditions, a conventional svFCS analysis suggests the presence of anomalous diffusion even though single molecule motions are nearly Brownian in these simulations. This misinterpretation is most significant when the surface density of the fluorescent label is elevated, therefore we suggest future measurements be made over a range of tracer densities. Some simulation conditions reproduce qualitative features of published svFCS experimental data. Overall, this work emphasizes the need to probe membranes using multiple complimentary experimental methodologies in order to draw conclusions regarding the nature of spatial and dynamical heterogeneity in these systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.