ArticleProceedings of the National Academy of Sciences of the United States of America2016
Pseudomonas syringae type III effector HopAF1 suppresses plant immunity by targeting methionine recycling to block ethylene induction.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 83 citations in OpenAlex.
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- Beyond the Halo: Molecular Mechanisms and Ecological Aspects of the Infection Strategy ofInternational journal of molecular sciences · 2026Review
- Role of Plasmodesmata During Plant-Pathogen Interactions.The plant pathology journal · 2026Review
- Pathogen effector tactics to suppress plant endomembrane system.Frontiers in plant science · 2026Review
- Cyclic-di-GMP controls Type III effector export and symptom development in Pseudomonas syringae infections via the export ATPase HrcN.PLoS pathogens · 2025Article
- Fungal oxalic acid inhibits the deamidation of CERK1 ectodomain to dampen chitin-triggered plant immunity.Science China. Life sciences · 2025Article
- RNA-binding proteins orchestrating immunity in plants.The Plant journal : for cell and molecular biology · 2025Review
- Plant Signaling Hormones and Transcription Factors: Key Regulators of Plant Responses to Growth, Development, and Stress.Plants (Basel, Switzerland) · 2025Review
- Plant Coping with Cold Stress: Molecular and Physiological Adaptive Mechanisms with Future Perspectives.Cells · 2025Review
- Plant microbiota feedbacks through dose-responsive expression of general non-self response genes.Nature plants · 2025Article
- Suppression of NLR-mediated plant immune detection by bacterial pathogens.Journal of experimental botany · 2023Review
- Plant microbiota controls an alternative root branching regulatory mechanism in plants.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Comparative sequence analysis of pPATH pathogenicity plasmids inFrontiers in plant science · 2023Article
- Fungal Effectoromics: A World in Constant Evolution.International journal of molecular sciences · 2022Review
- A pipeline of integrating transcriptome and interactome to elucidate central nodes in host-pathogens interactions.STAR protocols · 2022Article
- Action Mechanisms of Effectors in Plant-Pathogen Interaction.International journal of molecular sciences · 2022Review
- In planta transcriptomics reveals conflicts between pattern-triggered immunity and the AlgU sigma factor regulon.PloS one · 2022Article
- Article
- What the Wild Things Do: Mechanisms of Plant Host Manipulation by Bacterial Type III-Secreted Effector Proteins.Microorganisms · 2021Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
HopAF1 is a type III effector protein of unknown function encoded in the genomes of several strains of Pseudomonas syringae and other plant pathogens. Structural modeling predicted that HopAF1 is closely related to deamidase proteins. Deamidation is the irreversible substitution of an amide group with a carboxylate group. Several bacterial virulence factors are deamidases that manipulate the activity of specific host protein substrates. We identified Arabidopsis methylthioadenosine nucleosidase proteins MTN1 and MTN2 as putative targets of HopAF1 deamidation. MTNs are enzymes in the Yang cycle, which is essential for the high levels of ethylene biosynthesis in Arabidopsis We hypothesized that HopAF1 inhibits the host defense response by manipulating MTN activity and consequently ethylene levels. We determined that bacterially delivered HopAF1 inhibits ethylene biosynthesis induced by pathogen-associated molecular patterns and that Arabidopsis mtn1 mtn2 mutant plants phenocopy the effect of HopAF1. Furthermore, we identified two conserved asparagines in MTN1 and MTN2 from Arabidopsis that confer loss of function phenotypes when deamidated via site-specific mutation. These residues are potential targets of HopAF1 deamidation. HopAF1-mediated manipulation of Yang cycle MTN proteins is likely an evolutionarily conserved mechanism whereby HopAF1 orthologs from multiple plant pathogens contribute to disease in a large variety of plant hosts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.