ArticleScientific reports2016
An Unroofing Method to Observe the Cytoskeleton Directly at Molecular Resolution Using Atomic Force Microscopy.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- CryoGO enables high-resolution structural profiling of endogenous cellular macromolecules.bioRxiv : the preprint server for biology · 2026Article
- The effects of mechanical stimulation on the morphology and function of spinal ligament cells.BMC musculoskeletal disorders · 2025Article
- Advanced Freeze-Replica Methods for Virus Cell Biology.Methods in molecular biology (Clifton, N.J.) · 2025Article
- SPOT-RASTR-A cryo-EM specimen preparation technique that overcomes problems with preferred orientation and the air/water interface.PNAS nexus · 2024Article
- Simulating biological surface dynamics in high-speed atomic force microscopy experiments.Biophysical reviews · 2023Article
- A cryo-TSEM with temperature cycling capability allows deep sublimation of ice to uncover fine structures in thick cells.Scientific reports · 2021Article
- Actin Bundle Nanomechanics and Organization Are Modulated by Macromolecular Crowding and Electrostatic Interactions.Frontiers in molecular biosciences · 2021Article
- Nonlinear mechanics of lamin filaments and the meshwork topology build an emergent nuclear lamina.Nature communications · 2020Article
- Spatial high resolution of actin filament organization by PeakForce atomic force microscopy.Cell proliferation · 2020Article
- Visualization of internal in situ cell structure by atomic force microscopy.Histochemistry and cell biology · 2018Review
- BDNF Reduces eEF2 Phosphorylation and Enhances Novel Protein Synthesis in the Growth Cones of Dorsal Root Ganglia Neurons.Neurochemical research · 2018Article
- Morphological changes of plasma membrane and protein assembly during clathrin-mediated endocytosis.PLoS biology · 2018Article
- Biomechanical Characterization at the Cell Scale: Present and Prospects.Frontiers in physiology · 2018Review
- A Cryosectioning Technique for the Observation of Intracellular Structures and Immunocytochemistry of Tissues in Atomic Force Microscopy (AFM).Scientific reports · 2017Article
- Reversible differentiation of immortalized human bladder smooth muscle cells accompanied by actin bundle reorganization.PloS one · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An improved unroofing method enabled the cantilever of an atomic force microscope (AFM) to reach directly into a cell to visualize the intracellular cytoskeletal actin filaments, microtubules, clathrin coats, and caveolae in phosphate-buffered saline (PBS) at a higher resolution than conventional electron microscopy. All of the actin filaments clearly exhibited a short periodicity of approximately 5-6 nm, which was derived from globular actins linked to each other to form filaments, as well as a long helical periodicity. The polarity of the actin filaments appeared to be determined by the shape of the periodic striations. Microtubules were identified based on their thickness. Clathrin coats and caveolae were observed on the cytoplasmic surface of cell membranes. The area containing clathrin molecules and their terminal domains was directly visualized. Characteristic ridge structures located at the surface of the caveolae were observed at high resolution, similar to those observed with electron microscopy (EM). Overall, unroofing allowed intracellular AFM imaging in a liquid environment with a level of quality equivalent or superior to that of EM. Thus, AFMs are anticipated to provide cutting-edge findings in cell biology and histology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.