ArticleScientific reports2016
DNA Demethylation Rescues the Impaired Osteogenic Differentiation Ability of Human Periodontal Ligament Stem Cells in High Glucose.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 50 citations in OpenAlex.
- Mechanical forces orchestrate the epigenetic landscape of oral mesenchymal stem/progenitor cell fate in dental and periodontal tissues.Frontiers in cell and developmental biology · 2026Review
- Elimination of a neutrophil Pad4 byproduct restores stem cell-mediated bone regeneration in hyperglycemia.Nature communications · 2025Article
- Dichotomitin promotes osteoblast differentiation and improves osteoporosis by inhibiting oxidative stress.Journal of orthopaedic surgery and research · 2025Article
- Histone Deacetylase Inhibitors Restore the Odontogenic Differentiation Potential of Dental Pulp Stem Cells under Hyperglycemic Conditions.Current stem cell research & therapy · 2025Article
- Epigenetic control of dental stem cells: progress and prospects in multidirectional differentiation.Epigenetics & chromatin · 2024Review
- Dynamics of DNA methylation during osteogenic differentiation of porcine synovial membrane mesenchymal stem cells from two metabolically distinct breeds.Epigenetics · 2024Article
- Epigenetic changes underlie the association between diabetes mellitus and oral diseases.Molecular biology reports · 2023Review
- Dissecting specific Wnt components governing osteogenic differentiation potential by human periodontal ligament stem cells through interleukin-6.Scientific reports · 2023Article
- Article
- Osteogenesis of bone marrow mesenchymal stem cell in hyperglycemia.Frontiers in endocrinology · 2023Review
- DNA Methylation and Histone Modification in Dental-derived Mesenchymal Stem Cells.Stem cell reviews and reports · 2022Review
- Osteoporosis and Alveolar Bone Health in Periodontitis Niche: A Predisposing Factors-Centered Review.Cells · 2022Review
- Downregulation of DNA methyltransferase-3a ameliorates the osteogenic differentiation ability of adipose-derived stem cells in diabetic osteoporosis via Wnt/β-catenin signaling pathway.Stem cell research & therapy · 2022Article
- Probucol promotes osteoblasts differentiation and prevents osteoporosis development through reducing oxidative stress.Molecular medicine (Cambridge, Mass.) · 2022Article
- Article
- Epigenetic Regulation of Methylation in Determining the Fate of Dental Mesenchymal Stem Cells.Stem cells international · 2022Review
- Emerging role of epigenetic regulations in periodontitis: a literature review.American journal of translational research · 2022Review
- The Effect of Diabetes Mellitus on IGF Axis and Stem Cell Mediated Regeneration of the Periodontium.Bioengineering (Basel, Switzerland) · 2021Review
- Significant transcriptomic changes are associated with the inhibitory effects of 5-aza-2-deoxycytidine during adipogenic differentiation of MG-63 cells.Saudi journal of biological sciences · 2021Article
- SIRT6 Promotes Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells Through Antagonizing DNMT1.Frontiers in cell and developmental biology · 2021Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diabetes mellitus, characterized by abnormally high blood glucose levels, gives rise to impaired bone remodeling. In response to high glucose (HG), the attenuated osteogenic differentiation capacity of human periodontal ligament stem cells (hPDLSCs) is associated with the loss of alveolar bone. Recently, DNA methylation was reported to affect osteogenic differentiation of stem cells in pathological states. However, the intrinsic mechanism linking DNA methylation to osteogenic differentiation ability in the presence of HG is still unclear. In this study, we found that diabetic rats with increased DNA methylation levels in periodontal ligaments exhibited reduced bone mass and density. In vitro application of 5-aza-2'-deoxycytidine (5-aza-dC), a DNA methyltransferase inhibitor, to decrease DNA methylation levels in hPDLSCs, rescued the osteogenic differentiation capacity of hPDLSCs under HG conditions. Moreover, we demonstrated that the canonical Wnt signaling pathway was activated during this process and, under HG circumstances, the 5-aza-dC-rescued osteogenic differentiation capacity was blocked by Dickkopf-1, an effective antagonist of the canonical Wnt signaling pathway. Taken together, these results demonstrate for the first time that suppression of DNA methylation is able to facilitate the osteogenic differentiation capacity of hPDLSCs exposed to HG, through activation of the canonical Wnt signaling pathway.
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